BGT, T4 phage, Recombinant, His-Tag (DNA beta-glucosyltransferase, Beta-GT, beta-glucosyltransferase)
Catalog No : USB-137256
414.95€
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| Product name | BGT, T4 phage, Recombinant, His-Tag (DNA beta-glucosyltransferase, Beta-GT, beta-glucosyltransferase) | ||
|---|---|---|---|
| Catalog No | USB-137256 | ||
| Supplier’s Catalog No | 137256 | ||
| Supplier | US Biologicals | ||
| Source antigen | Recombinant, E. coli | ||
| Reactivity | |||
| Cross reactivity | |||
| Applications | |||
| Molecular weight | 41.6 | ||
| Storage | -70°C | ||
|---|---|---|---|
| Other names | |||
| Grade | Purified | ||
| Purity | Purified (~83%) | ||
| Form | Supplied as a liquid in 40mM Tris-HCl, pH 8.0, 110mM sodium chloride, 2.2mM potassium chloride, 200mM imidazole, 20% glycerol. | ||
| Reactivity life | 6 months | ||
| Note | For reserch purpose only | ||
| Purity | Purified (~83%) | ||
| Description | Catalyzes the transfer of glucose (Glc) from uridine diphosphoglucose (UDP-Glc) to 5-hydroxymethylcytosine (5-HMC) in double-stranded DNA. Is involved in a DNA modification process to protect the phage genome against its own nucleases and the host restriction endonuclease system. Source: Recombinant corresponding to aa1-351 from human BGT, T4 phage, fused to His-tag at C-terminal, expressed in E. coli. Molecular Weight: ~41.6kD Applications: Suitable for use in the differentiation of hydroxymethylcytosine (HMC) from methylcytosine in DNA, via glucosylating HMC and protecting HMC from endonuclease cleavage. Other applications not tested. Recommended Dilution: Optimal dilutions to be determined by the researcher. Storage and Stability: Aliquot to avoid repeated freezing and thawing and store at -70°C. Aliquots are stable for 6 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. | ||
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