BGT, T4 phage, Recombinant, His-Tag (DNA beta-glucosyltransferase, Beta-GT, beta-glucosyltransferase)

Catalog No : USB-137256
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Product name BGT, T4 phage, Recombinant, His-Tag (DNA beta-glucosyltransferase, Beta-GT, beta-glucosyltransferase)
Catalog No USB-137256
Supplier’s Catalog No 137256
Supplier US Biologicals
Source antigen Recombinant, E. coli
Reactivity
Cross reactivity
Applications
Molecular weight 41.6
Storage -70°C
Other names
Grade Purified
Purity Purified (~83%)
Form Supplied as a liquid in 40mM Tris-HCl, pH 8.0, 110mM sodium chloride, 2.2mM potassium chloride, 200mM imidazole, 20% glycerol.
Reactivity life 6 months
Note For reserch purpose only
Purity Purified (~83%)
Description Catalyzes the transfer of glucose (Glc) from uridine diphosphoglucose (UDP-Glc) to 5-hydroxymethylcytosine (5-HMC) in double-stranded DNA. Is involved in a DNA modification process to protect the phage genome against its own nucleases and the host restriction endonuclease system. Source: Recombinant corresponding to aa1-351 from human BGT, T4 phage, fused to His-tag at C-terminal, expressed in E. coli. Molecular Weight: ~41.6kD Applications: Suitable for use in the differentiation of hydroxymethylcytosine (HMC) from methylcytosine in DNA, via glucosylating HMC and protecting HMC from endonuclease cleavage. Other applications not tested. Recommended Dilution: Optimal dilutions to be determined by the researcher. Storage and Stability: Aliquot to avoid repeated freezing and thawing and store at -70°C. Aliquots are stable for 6 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.