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Categories: Biochemicals / Biochemicals

Product name			WB Antibody Stripping Buffer
Catalog No			BSP-BWR1063
Supplier’s Catalog No			BWR1063
Supplier			Biospes
Source antigen			N/A
Reactivity
Cross reactivity			None
Applications			WB
Molecular weight			N/A

Storage
Other names
Grade			Molecular biology
Purity			High
Form			Liquid
Reactivity life			12 months when stored at 4℃.
Note			For reserch purpose only
Description			WB Stripping Buffer is used for the recycle of deproteinized membrane in WB. In WB, after completing the conjugation of the primary and secondary antibodies as well as the later chemiluminescence, sometimes end user has to detect the proteins with stable expression level like Actin and Tubulin, etc. for reference, or test other proteins for comparison. But for this reagent, through dissociating the conjugation of primary, secondary antibodies and antigens, it removes primary and secondary antibodies from the membrane, then end user can reuse the same membrane to detect other proteins. Thus, compare with running a fresh SDS-PAGE gel, this reagent helps to eliminate the loading error and makes the experiment more comparable and accurate. For excluding the frequently-used β-Mercaptoethanol, this reagent is nontoxic, tasteless and harmless, can be used at room temperature. This reagent is enough for at least 5 times WB detections on the same membrane, and the whole detection can be completed within15-30 min.