Transferrin, Human, Iron-Free (APO)
Catalog No : USB-299296
508.06€
0.00€
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| Product name | Transferrin, Human, Iron-Free (APO) | ||
|---|---|---|---|
| Catalog No | USB-299296 | ||
| Supplier’s Catalog No | 299296 | ||
| Supplier | US Biologicals | ||
| Source antigen | Plasma | ||
| Reactivity | |||
| Cross reactivity | |||
| Applications | |||
| Molecular weight | 77 | ||
| Storage | -20°C | ||
|---|---|---|---|
| Other names | |||
| Grade | Highly Purified | ||
| Purity | ~98% (Cellulose Acetate Electrophoresis) | ||
| Form | Supplied as a lyophilized powder in 0.02M NH4HCO3. May contain traces of buffer salts. Reconstitute with 100ug/ml sterile ddH2O. | ||
| Reactivity life | 12 months | ||
| Note | For reserch purpose only | ||
| Purity | ~98% (Cellulose Acetate Electrophoresis) | ||
| Description | Transferrin is the iron-transport protein of vertebrate serum and donates iron to cells through interaction with a specific membrane receptor, CD71. Transferrin appears to be indispensable for most cells growing in tissue culture. It is referred to frequently as a growth factor because, in analogy to other growth factor-receptor interactions, proliferating cells express high numbers of transferrin receptors, and the binding of transferrin to their receptors is needed for cells to initiate and maintain their DNA synthesis. Apart from its role as an iron transport protein transferrin acts as a cytokine and has functions that may not be related to its iron-carrying capacity. Source: Human Apo Transferrin, from Plasma (FDA approved) Form: Supplied as a lyophilized powder in 0.02M NH4HCO3. May contain traces of buffer salts. Reconstitute with 100ug/ml sterile ddH2O. Purity: ≥98% (Cellulose Acetate Electrophoresis) Human Virus Test: FDA approved Plasma from each donor has been tested and found negative for antibody to HIV-1, HIV-2, HCV, HBSAG, HBc, ALT and Syphilis. Viral inactivation by pasteurization (60°C for 10 hours) has been validated using three different test viruses, with removal of 8-14.5 logs of virus documented. The purification process has also been found to remove significant additional quantities of virus. Applications: Suitable for use in the cultivation and long-term cells growth of mammalian cells in vitro, as a nutrient in fermentation media for recombinant protein and biopharmaceutical production. Additional common uses of human Transferrin are molecular weight, Affinity purification of anti-human transferrin antibodies and receptor mediated transfection of molecules such as DNA, into cells. Other applications not tested. Storage and Stability: Lyophilized powder may be stored at -20°C. Stable for 12 months after receipt at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. | ||
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