Imugex

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Categories: Peptides & Proteins / Proteins

Product name			Protein Synthesis Initiation Factor 4E, Recombinant, Human (eIF 4E, Eukaryotic Translation Initiation Factor)
Catalog No			USB-P9108-50
Supplier’s Catalog No			P9108-50
Supplier			US Biologicals
Source antigen			Human recombinant kidney.
Reactivity
Cross reactivity
Applications
Molecular weight			28

Storage			-70°C
Other names
Grade			Highly Purified
Purity			≥ 90% (SDS-PAGE)
Form			Supplied as a liquid in 50mM Tris HCl, pH 7.0, b-mercaptoethanol, benzamidine, PMSF, EDTA, 10% glycerol.
Reactivity life			6 months
Note			For reserch purpose only
Purity			≥ 90% (SDS-PAGE)
Description			Eukaryotic initiation factor 4E (eIF4E) binds to the mRNA cap structure, thereby mediating the initiation of translation (1,2). eIF4E interacts with eIF4G, which serves as a scaffold protein for the assembly of eIF4E and eIF4A to form the eIF4F complex (2). Upon activation by mitogenic and/or stress stimuli mediated by Erk and p38 MAPK, Mnk1 has been shown to phosphorylate eIF4E at Ser209 in vivo (3,4). Two Erk and p38 MAPK phosphorylation sites have been identified in mouse Mnk1, Thr197 and Thr202, which are essential for Mnk1 kinase activity (3). The C-terminal region of eIF4G also contains serum-stimulated phosphorylation sites, including Ser1108, Ser1148 and Ser1192 (5). It is known that their phosphorylation is blocked by the PI3 kinase inhibitor LY294002 and by the FRAP/mTOR inhibitor rapamycin. Storage and Stability: Aliquot to avoid repeated freezing and thawing and store at -70°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquots are stable for at least 12 months.