eIF5A, Recombinant, Human, aa1-154 (Eukaryotic translation initiation factor 5A Isoform B, EIF-5A, EIF5A1, eIF5AI)

Catalog No : USB-E8950-01B
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Product name eIF5A, Recombinant, Human, aa1-154 (Eukaryotic translation initiation factor 5A Isoform B, EIF-5A, EIF5A1, eIF5AI)
Catalog No USB-E8950-01B
Supplier’s Catalog No E8950-01B
Supplier US Biologicals
Source antigen Recombinant, E. coli
Reactivity
Cross reactivity
Applications
Molecular weight 16.8
Storage -20°C
Other names
Grade Highly Purified
Purity ~95% (SDS-PAGE)
Form Supplied as liquid in 50mM Tris-HCl, pH 7.5, 10% glycerol.
Reactivity life 6 months
Note For reserch purpose only
Purity ~95% (SDS-PAGE)
Description Eukaryotic translation initiation factor 5A (eIF5A) is the only protein known to contain unusual amino acid formed by the action of deoxyhypusine synthase and deoxyhypusine hydroxylase using spermidine as the substrate. This protein was previously reported to be involved in the first step of peptide bond formation in translation; however more recent work implicates it as a universally conserved translation elongation factor. Modulation of eIF5A has been linked to proliferation and cancer. Source: Recombinant human eIF5A protein was expressed in E. coli and purified by using conventional chromatography techniques. AA Sequence: MADDLDFETG DAGASATFPM QCSALRKNGF VVLKGRPCKI VEMSTSKTGK HGHAKVHLVG IDIFTGKKYE DICPSTHNMD VPNIKRNDFQ LIGIQDGYLS LLQDSGEVRE DLRLPEGDLG KEIEQKYDCG EEILITVLSA MTEEAAVAIK AMAK Molecular Weight: 16.8kD (154aa) Storage and Stability: May be stored at 4°C for short-term only. For long-term storage, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.