Eukaryotic Translation Initiation Factor 4 Gamma 1, Recombinant, Human, aa1250-1599, His-B2M-JD-Tag, Myc-Tag (EIF4G1)

Catalog No : USB-517896
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Product name Eukaryotic Translation Initiation Factor 4 Gamma 1, Recombinant, Human, aa1250-1599, His-B2M-JD-Tag, Myc-Tag (EIF4G1)
Catalog No USB-517896
Supplier’s Catalog No 517896
Supplier US Biologicals
Source antigen Recombinant, E. coli
Reactivity
Cross reactivity
Applications
Molecular weight 46.6
Storage -20°C
Other names
Grade Purified
Purity ~85% (SDS-PAGE)
Form Supplied as a liquid in Tris-based buffer, 50% glycerol.
Reactivity life
Note For reserch purpose only
Purity ~85% (SDS-PAGE)
Description Component of the protein complex eIF4F, which is involved in the recognition of the mRNA cap, ATP-dependent unwinding of 5'-terminal secondary structure and recruitment of mRNA to the ribosome. Source: Partial recombinant protein corresponding to aa1250-1599 of human Eukaryotic Translation Initiation Factor 4 Gamma 1, fused to His-B2M-JD-Tag at N-terminal and Myc-Tag at C-terminal, expressed in E. coli. Molecular Weight: ~46.6kD AA Sequence: IEEYLHLNDMKEAVQCVQELASPSLLFIFVRHGVESTLERSAIAREHMGQLLHQLLCAGHLSTAQYYQGLYEILELAEDMEIDIPHVWLYLAELVTPILQEGGVPMGELFREITKPLRPLGKAASLLLEILGLLCKSMGPKKVGTLWREAGLSWKEFLPEGQDIGAFVAEQKVEYTLGEESEAPGQRALPSEELNRQLEKLLKEGSSNQRVFDWIEANLSEQQIVSNTLVRALMTAVCYSAIIFETPLRVDVAVLKARAKLLQKYLCDEQKELQALYALQALVVTLEQPPNLLRMFFDALYDEDVVKEDAFYSWESSKDPAEQQGKGVALKSVTAFFKWLREAEEESDHN Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 6 months after receipt at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.