Glutamate Transporter, Aspartate EAAT1, Rat (GLAST) Control Peptide
Catalog No : USB-G4003-90A
417.25€
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| Product name | Glutamate Transporter, Aspartate EAAT1, Rat (GLAST) Control Peptide | ||
|---|---|---|---|
| Catalog No | USB-G4003-90A | ||
| Supplier’s Catalog No | G4003-90A | ||
| Supplier | US Biologicals | ||
| Source antigen | Rat synthetic peptide | ||
| Reactivity | Synthetic peptide (15) near the C-terminus of rat GLAST. No significant sequence homology to other glutamate transporters. Species Sequence Homology: Mouse, human and bovine: 100%; chicken and canine: 93%. | ||
| Cross reactivity | |||
| Applications | |||
| Molecular weight | |||
| Storage | -20°C | ||
|---|---|---|---|
| Other names | |||
| Grade | Purified | ||
| Purity | Purified | ||
| Form | Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide. | ||
| Reactivity life | 12 months | ||
| Note | For reserch purpose only | ||
| Purity | Purified | ||
| Description | Control peptide for G4003-90 (affinity purified antibody) and G4003-90B (antiserum). Glutamate is the main excitatory neurotransmitter in the brain. To date five glutamate Transporters have been cloned: GLAST (EAAT1), GLT1 (EAAT2), EAAC1 (EAAT3), EAAT4 and EAAT5. These transporters are believed to be critical in reducing potentially toxic extracellular concentration of glutamate by rapid uptake into nerve terminals and glial cells. Glutamate transporters (525-573aa) display about 55% homology. They are predicted to contain up to 6-10 transmembrane domains. Immunolocalization studies indicate that GLT1 is localized in astroglial cells throughout the brain and spinal cord. EAAC1 is specific for certain neurons and Purkinje cells. It is specifically enriched in cortex, hippocampus and caudate-putamen. It is confined to presynaptic and postsynaptic elements. GLAST has been observed in both neuron and astroglia. It is most abundant in Bergmann glia, cortex, hippocampus and cerebellum. EAAT4 has properties of ligand gated Cl-channel. It is localized mainly in cerebellar Purkinje cells in rat and human CNS. EAAT5 has only been cloned from human. It is primarily expressed in retina. GLAST (sodium dependent Glutamate/Aspartate Transporter 1, or Excitatory Amino Acid Transporter 1, EAAT1) is a glutamate transporter. It is a transmembrane protein with ~543aa and a molecular weight of 67kD. Applications: Suitable for use in ELISA, Antibody Blocking. Not suitable for use in Western Blot due to its loq molecular weight. Other applications not tested. Recommended Dilution: Antibody Blocking: 5-10ug per 1ug G4003-90 (affinity purified antibody) or 1ul G4003-90B (antiserum). ELISA: 0.5-1ug/ml. Control peptide can be used to coat ELISA plates at 1ug/ml. Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. | ||
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