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Categories: Peptides & Proteins / Proteins

Product name			ARF1, Recombinant, Human (ADP-ribosylation Factor 1)
Catalog No			USB-A0901-81
Supplier’s Catalog No			A0901-81
Supplier			US Biologicals
Source antigen			Recombinant, E.coli
Reactivity
Cross reactivity
Applications
Molecular weight			22.8

Storage			-20°C
Other names
Grade			Affinity Purified
Purity			~90% (SDS-PAGE).
Form			Supplied as a liquid in 20mM Tris-HCl, pH 8.0, 20% glycerol, 0.1M sodium chloride, 1mM DTT.
Reactivity life			6 months
Note			For reserch purpose only
Purity			~90% (SDS-PAGE).
Description			ARF1, also known as ADP-ribosylation factor 1, is a small guanine nucleotide-binding protein that enhances the enzymatic activities of cholera toxin. It is essential and ubiquitous in eukaryotes, as this protein is involved in vesicular transport and functioning via phospholipase D activation. ARF1 plays a role in membrane traffic and organelle integrity and are intimately tied to their reversible association with membranes and distinct interactions with membrane phospholipids. Source: Recombinant corresponding to aa1-181 of human ARF1 protein, fused to His-tag at N-terminus, expressed in E.coli. AA Sequence: MGSSHHHHHH SSGLVPRGSH MGNIFANLFK GLFGKKEMRI LMVGLDAAGK TTILYKLKLG EIVTTIPTIG FNVETVEYKN ISFTVWDVGG QDKIRPLWRH YFQNTQGLIF VVDSNDRERV NEAREELMRM LAEDELRDAV LLVFANKQDL PNAMNAAEIT DKLGLHSLRH RNWYIQATCA TSGDGLYEGL DWLSNQLRNQ K Molecular Weight: 22.8kD (201aa) confirmed by MALDI-TOF Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.