TFF1, Recombinant, Human (Trefoil Factor 1, Breast Cancer Estrogen-inducible Protein, PNR-2, Polypeptide P1.A, hP1.A, Protein pS2, BCEI, PS2, D21S21, hP1.A, HP1.A)

Catalog No : USB-143513
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Product name TFF1, Recombinant, Human (Trefoil Factor 1, Breast Cancer Estrogen-inducible Protein, PNR-2, Polypeptide P1.A, hP1.A, Protein pS2, BCEI, PS2, D21S21, hP1.A, HP1.A)
Catalog No USB-143513
Supplier’s Catalog No 143513
Supplier US Biologicals
Source antigen Recombinant, E. coli
Reactivity
Cross reactivity
Applications
Molecular weight
Storage -20°C
Other names
Grade Highly Purified
Purity ≥98% by SDS-PAGE gel and HPLC analyses.
Form Supplied as a lyophilized powder.
Reactivity life 12 months
Note For reserch purpose only
Purity ≥98% by SDS-PAGE gel and HPLC analyses.
Description The Trefoil Factor peptides (TFF1, TFF2 and TFF3) are expressed in the gastrointestinal tract, and appear to play an important role in intestinal mucosal defense and repair. TFF1 is essential for normal differentiation of the antral and pyloric gastric mucosa and functions as a gastric-specific tumor suppressor gene. Recombinant human TFF1 is a 6.7kD monomeric protein consisting of a 60aa polypeptide chain, which includes a 40aa trefoil motif containing three conserved intramolecular disulfide bonds. Biological Activity: Determined by its ability to chemoattract human MCF-7 cells using a concentration 5-10ug/ml. AA Sequence: EAQTETCTVA PRERQNCGFP GVTPSQCANK GCCFDDTVRG VPWCFYPNTI DVPPEEECEF Quality Control: Verified by N-terminal and Mass Spectrometry analyses (when applicable). Endotoxin: <0.1ng/ug of protein (<1EU/ug). Protein Content: Verified by UV Spectroscopy and/or SDS-PAGE gel. Storage and Stability: Store lyophilized products at -20°C. For reconstituted solutions of most products, we recommend short-term storage at 4°C. For longer term storage the protein solution should be stored with a carrier protein (eg. 0.1% BSA) in working aliquots and stored frozen at -20°C. Additional freeze/thaw cycles may cause some denaturation of the protein.