SCFB-TrCP1 Complex, Active, Recombinant, Human (SCF-B-TrCP, SCFFWD10)
Catalog No : USB-029605
793.13€
0.00€
Shipping cost plus VAT not included , delivery in 7-14 business days
| Product name | SCFB-TrCP1 Complex, Active, Recombinant, Human (SCF-B-TrCP, SCFFWD10) | ||
|---|---|---|---|
| Catalog No | USB-029605 | ||
| Supplier’s Catalog No | 029605 | ||
| Supplier | US Biologicals | ||
| Source antigen | Recombinant, Sf21 | ||
| Reactivity | |||
| Cross reactivity | |||
| Applications | |||
| Molecular weight | 191146193112 | ||
| Storage | -70°C | ||
|---|---|---|---|
| Other names | |||
| Grade | Highly Purified | ||
| Purity | ~92% (SDS-PAGE) | ||
| Form | Supplied as a liquid in 50mM Tris-HCl, pH 7.5, 300mM sodium chloride, 0.1mM EGTA, 0.03% Brij-35, 270mM sucrose, 1mM benzamidine, 0.2mM PMSF, 0.1% 2- mercaptoethanol. | ||
| Reactivity life | 6 months | ||
| Note | For reserch purpose only | ||
| Purity | ~92% (SDS-PAGE) | ||
| Description | The SCF complexes represent the largest family of ubiquitin-protein ligases and mediate the ubiquitination of a broad spectrum of regulatory and signalling proteins in diverse cellular pathways. The SCF consists of three invariant components, Skp1, Cul1, and Rbx1 and an interchangeable subunit, an F-box protein which is responsible for recruiting specific substrates to be ubiquitinated by the SCF. SCFBTrCP1 mediates the ubiquitination and subsequent proteasomal degradation of a variety of proteins involved in various cellular processes, these include IkBa, an inhibitor of the transcription factor NFkB and B-catenin which is involved in the Wnt signalling pathway. A number of cancers, including those in breast, colon and pancreatic tissue have been associated with the deregulation of SCFBTrCP1. Source: Recombinant protein corresponding to complex of N-terminal GST- tagged, recombinant human bTrCP1 aa53-end, N-terminal GST-tagged, recombinant human Skp1 full length, N-terminal 6His-tagged, recombinant human Cul1 full length and untagged recombinant human Rbx1 full length, co-expressed by baculovirus in Sf21 insect cells. Molecular Weight: bTrCP1: 91kD, Skp1: 46kD, Cul1: 93kD, Rbx1: 12kD Storage and Stability: Aliquot to avoid repeated freezing and thawing and store at -70°C. Stable for 6 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. | ||
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