Syk, active, Recombinant, Human
Catalog No : USB-S9100-08
718.41€
0.00€
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| Product name | Syk, active, Recombinant, Human | ||
|---|---|---|---|
| Catalog No | USB-S9100-08 | ||
| Supplier’s Catalog No | S9100-08 | ||
| Supplier | US Biologicals | ||
| Source antigen | Recombinant, Human from Sf21 | ||
| Reactivity | |||
| Cross reactivity | |||
| Applications | |||
| Molecular weight | 73 | ||
| Storage | -70°C | ||
|---|---|---|---|
| Other names | |||
| Grade | Purified | ||
| Purity | ~60% by SDS-PAGE and Coomassie blue staining. Purified using Ni2+/NTA-agarose. | ||
| Form | Supplied as a liquid in 50mM Tris-HCl, pH 7.5, 150mM sodium chloride, 270mM sucrose, 0.1mM EGTA, 0.1% 2-mercaptoethanol, 0.03% Brij-35, 1mM benzamidine, 0.2mM PMSF. | ||
| Reactivity life | 6 months | ||
| Note | For reserch purpose only | ||
| Purity | ~60% by SDS-PAGE and Coomassie blue staining. Purified using Ni2+/NTA-agarose. | ||
| Description | Source: N-terminus, His-tagged fusion protein corresponding to full-length human Syk as confirmed by Ms tryptic fingerprinting and protein sequencing, expressed in Sf21. Applications: Suitable for use in MS Tryptic Fingerprint and Kinase Assay. Other applications not tested. Recommended Dilution: MS Tryptic Fingerprint: Confirmed identity as Syk with translated sequence. Kinase Assay: 20-55ng phosphorylated 0.1mg/ml poly(Glu, Tyr) (4:1). Optimal dilutions to be determined by the researcher. Specific Activity: 195U/mg; One unit of Syk activity is defined as 1nmol phosphate incorporated into 0.1mg/ml poly(Glu, Tyr) (4:1) per minute at 30ºC with a final ATP concentration of 100uM. Storage and Stability: Aliquot to avoid repeated freezing and thawing and store at -70°C. Aliquots are stable for 6 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Handling Recommendations: Rapidly thaw the vial under cold water and immediately place on ice. Aliquot unused material into pre-chilled microcentrifuge tubes and immediately snap-freeze the vials in liquid nitrogen prior to re-storage at -70°C. | ||
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