Flt3 Ligand, Recombinant, Mouse (Fms-like Tyrosine Kinase 3 Ligand, flk2 Ligand)
Catalog No : USB-F4600-15
377.02€
0.00€
Shipping cost plus VAT not included , delivery in 7-14 business days
| Product name | Flt3 Ligand, Recombinant, Mouse (Fms-like Tyrosine Kinase 3 Ligand, flk2 Ligand) | ||
|---|---|---|---|
| Catalog No | USB-F4600-15 | ||
| Supplier’s Catalog No | F4600-15 | ||
| Supplier | US Biologicals | ||
| Source antigen | Recombinant, Murine (E. coli) | ||
| Reactivity | |||
| Cross reactivity | |||
| Applications | |||
| Molecular weight | |||
| Storage | -20°C | ||
|---|---|---|---|
| Other names | |||
| Grade | Highly Purified | ||
| Purity | ≥ 98% by RP-HPLC, anion-exchange FPLC, or reducing/non-reducing SDS-PAGE Silver Stain. Endotoxin: ≤ 0.1ng/ug (1EU/ug) | ||
| Form | Supplied as a white lyophilized powder with no additives. Reconstitute with sterile ddH2O, 0.1% HSA or BSA to ≥0.1mg/ml | ||
| Reactivity life | 6 months | ||
| Note | For reserch purpose only | ||
| Purity | ≥ 98% by RP-HPLC, anion-exchange FPLC, or reducing/non-reducing SDS-PAGE Silver Stain. Endotoxin: ≤ 0.1ng/ug (1EU/ug) | ||
| Description | Human FLT3 ligand also stimulates the proliferation of cells expressing murine FLT-3 receptors. The effects of FLT-3 ligand are synergized by coexpression of G-CSF , GM-CSF , M-CSF , IL3 and SCF . FLT-3 ligand promotes long-term expansion and differentiation of human pro-B-cells in the presence of IL7 or in combination of IL7 and IL3 . Recombinant Murine Flt3-Ligand is a non-glycosylated, polypeptide chain containing 144 amino acids with a MW of 16.43kD. Sequence: The sequence of the first five N-terminal amino acids was determined and was found to be Thr-Gln-Asp-Cys-Ser. Dimers and Aggregates: ≤ 1% as determined by silver-stained SDS-PAGE gel analysis. Protein Content: Protein quantitation was carried out by two independent methods: 1. UV spectroscopy at 280nm. 2. Analysis by RP-HPLC, using a standard solution of FLT3 as a Reference Standard. Biological Activity: rmFlt3-Ligand is fully biologically active when compared to standard. The activity is determined by the ability to generate CD11c+ splenic dendritic cells in vivo in C57B1/6-12 week female mice. To obtain the desired affect, mice were treated with approximately 25ug of flt3-ligand once daily for 9 consecutive days via intraperitoneal injection. Storage and Stability: Lyophilized powder may be stored at 4°C for short-term only. For long-term storage, store below -20°C. Reconstitute to nominal volume by adding sterile ddH2O, 0.1% HSA or BSA and store below -20°C. Reconstituted product is stable for 12 months below -20°C. Avoid freeze-thaw cycles. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made in assay buffer. | ||
Product Details
© 2020 Imugex All Rights Reserved