BMX, Active, Recombinant, Human (Cytoplasmic Tyrosine-protein Kinase BMX, Bone Marrow Tyrosine Kinase Gene in Chromosome X Protein, ETK, Epithelial and Endothelial Tyrosine Kinase, NTK38)
Catalog No : USB-219478
560.94€
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| Product name | BMX, Active, Recombinant, Human (Cytoplasmic Tyrosine-protein Kinase BMX, Bone Marrow Tyrosine Kinase Gene in Chromosome X Protein, ETK, Epithelial and Endothelial Tyrosine Kinase, NTK38) | ||
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| Catalog No | USB-219478 | ||
| Supplier’s Catalog No | 219478 | ||
| Supplier | US Biologicals | ||
| Source antigen | Recombinant, Baculovirus (Sf9 insect cells) | ||
| Reactivity | |||
| Cross reactivity | |||
| Applications | |||
| Molecular weight | 110 | ||
| Storage | -70°C | ||
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| Other names | |||
| Grade | Purified | ||
| Purity | ~80% (SDS-PAGE) | ||
| Form | Supplied as a liquid in 50mM Tris-HCl, pH 7.5, 150mM sodium chloride, 0.25mM DTT, 0.1mM EGTA, 0.1mM EDTA, 0.1mM PMSF, 25% glycerol. | ||
| Reactivity life | 12 months | ||
| Note | For reserch purpose only | ||
| Purity | ~80% (SDS-PAGE) | ||
| Description | The BMX gene encodes a novel non receptor tyrosine kinase, which may play a role in the growth and differentiation of hematopoietic cells. Bmx cDNA comprises a long open reading frame of 675aa, containing one SH3, one SH2 and one tyrosine kinase domain, which are about 70% identical with Btk, Itk and Tec and somewhat less with Txk tyrosine kinase sequences. The amino terminal sequences of these four tyrosine kinases are about 40% identical and each contains a so-called pleckstrin homology (PH) domain. The 2.7kb Bmx mRNA is expressed in endothelial cells and several human tissues by Northern blotting and an 80kD Bmx polypeptide was detected in human endothelial cells. The BMX gene is located in chromosomal band Xp22.2 between the DXS197 and DXS207 loci. Interestingly, chromosome X also contains the closest relative of BMX, the BTK gene, implicated in X-linked ayglobulinemia. Bmx, is found to induce activation of the Stat signaling pathway. Bmx induced the tyrosine phosphorylation and DNA binding activity of all the Stat factors tested, including Stat1, Stat3, and Stat5, both in mammalian and insect cells. Bmx also induced transcriptional activation of Stat1- and Stat5-dependent reporter genes. Source: Recombinant protein corresponding to active human BMX, expressed in Baculovirus (Sf9 insect cells). Molecular Weight: ~110kD Biological Activity: ~29nmol phosphate incorporated into Poly Glu-Tyr substrate/minute/mg protein at 30°C, using a final concentration of 50uM ATP (0.83uCi/assay). Storage and Stability: Aliquot to avoid repeated freezing and thawing and store at -70°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. | ||
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