Flt3 Ligand, Recombinant, Human (Fms-like Tyrosine Kinase 3 Ligand, flk2 Ligand)

Catalog No : USB-219771
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Product name Flt3 Ligand, Recombinant, Human (Fms-like Tyrosine Kinase 3 Ligand, flk2 Ligand)
Catalog No USB-219771
Supplier’s Catalog No 219771
Supplier US Biologicals
Source antigen Recombinant, E. coli
Reactivity
Cross reactivity
Applications
Molecular weight 17.6
Storage -20°C
Other names
Grade Highly Purified
Purity ~98% (SDS-PAGE, HPLC)
Form Supplied as a lyophilized powder. No preservative added. Reconstitute with ddH2O to 0.1-1.0mg/ml.
Reactivity life 12 months
Note For reserch purpose only
Purity ~98% (SDS-PAGE, HPLC)
Description Human flt3-Ligand (flk2-ligand) stimulates the proliferation and colony formation of certain bone marrow precursor cells including CD34+ cells. Recombinant human flt3-ligand is a soluble polypeptide containing 155aa residues, which comprises the extracellular domain of the transmembrane flt3-ligand protein. Source: Recombinant protein corresponding to human flt3-Ligand expressed in E. coli. Molecular Weight: ~17.6kD Biological Activity: The ED50, as determined by the dose-dependent stimulation of the proliferation of human AML5 cells, is <1.0ng/ml, corresponding to a specific activity of >1x10e6units/mg. Endotoxin: <0.1ng/ug Storage and Stability: Lyophilized powder may be stored at -20°C. Stable for 12 months after receipt at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.