Sentrin 1, Recombinant, Human (Small Ubiquitin-related Modifier 1, SUMO 1, SMT3C)
Catalog No : USB-S0750-01F
377.02€
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| Product name | Sentrin 1, Recombinant, Human (Small Ubiquitin-related Modifier 1, SUMO 1, SMT3C) | ||
|---|---|---|---|
| Catalog No | USB-S0750-01F | ||
| Supplier’s Catalog No | S0750-01F | ||
| Supplier | US Biologicals | ||
| Source antigen | Recombinant, E. coli | ||
| Reactivity | |||
| Cross reactivity | |||
| Applications | |||
| Molecular weight | |||
| Storage | -20°C | ||
|---|---|---|---|
| Other names | |||
| Grade | Highly Purified | ||
| Purity | ≥ 95% by RP-HPLC, FPLC, or reducing/non-reducing SDS-PAGE Silver Stain. Chromatographically purified. | ||
| Form | Supplied as a lyophilized powder. No additives. | ||
| Reactivity life | 6 months | ||
| Note | For reserch purpose only | ||
| Purity | ≥ 95% by RP-HPLC, FPLC, or reducing/non-reducing SDS-PAGE Silver Stain. Chromatographically purified. | ||
| Description | Active human SUMO-I (the 1-97 amino acid region of the Ubiquitin-like protein SMT3C precursor also named Ubiquitin-homology domain protein PIC1, Ubiquitin-like protein UBL1, Ubiquitin-related protein SUMO-I, GAP modifying protein-I, GMP-I, and Sentrin-I. Human SUMO-1 (small ubiquitin-like modifier) is an 11kD protein that shares 18% sequence homology with ubiquitin. Smt3p, which is 48% identical to human SUMO-1, has been identified in yeast. SUMO-1/Smt3p is activated by a heterodimeric E1 enzyme in both the yeast and mammalian systems It has been shown that SUMO-1 can be conjugated to a number of proteins such as PML, RanGAP1, I B, FAS/apolipoprotein-1, p53, RAD51 and RAD52. Recombinant Human SUMO1 is a single, non-glycosylated, polypeptide chain having a molecular mass of 20kD as seen on SDS-PAGE. Endotoxin: ≤ 0.1ng/ug (IEU/ug) of ubiquitin-conjugating enzyme Storage and Stability: May be stored at 4°C for short-term only. For long-term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. | ||
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