Ubc9, Recombinant, Human (Ubiquitin Conjugating Enzyme 9)
Catalog No : USB-U0880-06
348.29€
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| Product name | Ubc9, Recombinant, Human (Ubiquitin Conjugating Enzyme 9) | ||
|---|---|---|---|
| Catalog No | USB-U0880-06 | ||
| Supplier’s Catalog No | U0880-06 | ||
| Supplier | US Biologicals | ||
| Source antigen | Recombinant, E. coli | ||
| Reactivity | |||
| Cross reactivity | |||
| Applications | |||
| Molecular weight | |||
| Storage | -20°C | ||
|---|---|---|---|
| Other names | |||
| Grade | Highly Purified | ||
| Purity | ≥ 95% by RP-HPLC, FPLC, or reducing/non-reducing SDS-PAGE Silver Stain. Chromatographically purified. | ||
| Form | Supplied as a liquid in 10mM Tris-HCl, pH 7.5, 100mM sodium chloride, 100mM imidazole, 0.5mM PMSF, 1mM DTT, 10% glycerol. | ||
| Reactivity life | 6 months | ||
| Note | For reserch purpose only | ||
| Purity | ≥ 95% by RP-HPLC, FPLC, or reducing/non-reducing SDS-PAGE Silver Stain. Chromatographically purified. | ||
| Description | Human UBC9 Ubiquitin-like protein SUMO-1 conjugating enzyme is also called UBE2I. rHuUBC9 Mediates the conjugation of the ubiquitin-like protein SUMO-1 to a variety of proteins including RanGAP1, IkappaB alpha, and PML without the requirement of an ubiquitin ligase (E3). Recombinant Human ubiquitin-conjugating enzyme is a single, non-glycosylated, polypeptide chain having a molecular mass of 23kD. Dimers and Aggregates: ≤ 1% as determined by silver-stained SDS-PAGE gel analysis. Biological Activity: IGF-I is fully biologically active when compared to standards. The ED50, calculated by the dose-dependent proliferation of murine BALB\C 3T3 cells (measured by H-thymidine uptake) is less then 1.0ng/ml, corresponding to a specific activity of 1x10e6 U/mg. For most in-vitro applications, IGF-I exerts its biological activity in the concentration range of 0.2-20ng/ml. Endotoxin: ≤ 0.1ng/ug (IEU/ug) of ubiquitin-conjugating enzyme Protein Content: Protein quantitation was carried out by two independent methods: 1. UV spectroscopy at 280nm 2. Analysis by RP-HPLC, using a standard solution of UBC-9 as a Reference Standard. Storage and Stability: May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. | ||
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