Ubc9, Recombinant, Human (Ubiquitin Conjugating Enzyme 9)
Catalog No : USB-U0880-05A
377.02€
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| Product name | Ubc9, Recombinant, Human (Ubiquitin Conjugating Enzyme 9) | ||
|---|---|---|---|
| Catalog No | USB-U0880-05A | ||
| Supplier’s Catalog No | U0880-05A | ||
| Supplier | US Biologicals | ||
| Source antigen | Recombinant, E. coli | ||
| Reactivity | |||
| Cross reactivity | |||
| Applications | |||
| Molecular weight | 17.9 | ||
| Storage | -20°C | ||
|---|---|---|---|
| Other names | |||
| Grade | Highly Purified | ||
| Purity | ≥95% by RP-HPL and reducing/ non-reducing SDS-PAGE (silver stained). Endotoxin: ≤0.1ng/ug (IEU/ug) | ||
| Form | Supplied as a Lyophillized powder in PBS, pH 7.5, 1mM DTT. Reconstitute with sterile ddH2O, 0.1% HSA or BSA to a concentration ≥0.1mg/ml. | ||
| Reactivity life | 6 months | ||
| Note | For reserch purpose only | ||
| Purity | ≥95% by RP-HPL and reducing/ non-reducing SDS-PAGE (silver stained). Endotoxin: ≤0.1ng/ug (IEU/ug) | ||
| Description | Human Ubc9 is homologous to ubiquitin-conjugating enzymes(E2s). However, instead of conjugating ubiquitin, it conjugates a ubiquitin homologue, small ubiquitin-like modifier 1(SUMO-1). And hUbc9 retains striking structural and functional conservation with yeast Ubc9. The ubiquitin-dependent protein degradation system has been recognized as a complete enzymatic pathway that is responsible for the selective degradation of abnormal and short-lived proteins. The conjugation of ubiquitin requires the activities of ubiquitin-activating(E1) and –conjugating(E2) enzymes. Recombinant Human UbC9 produced in E.Coli is a single, non-glycosylated polypeptide chain containing 154 amino acids & having a molecular mass of 17.9kD. Sequence: MSGIALSRLA QERKAWRKDH PFGFVAVPTK NPDGTMNLMN WECAIPGKKG TPWEGGLFKL RMLFKDDYPS SPPKCKFEPP LFHPNVYPSG TVCLSILEED KDWRPAITIK QILLGIQELL NEPNIQDPAQ AEAYTIYCQN RVEYEKRVRA QAKKFAPS Storage and Stability: Lyophilized powder may be stored at 4°C for short-term only. Reconstitute to nominal volume by adding with sterile dH2O, 0.1% HSA or BSA, aliquot and store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. | ||
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