Imugex

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Categories: Peptides & Proteins / Proteins

Product name			Ubiquitin Activating Enzyme 6, N-terminal FLAG-Tag, Recombinant, Human (UBA6, UBE1L2, E1-L2, FLJ10808, FLJ23367, Monocyte protein 4, MOP4, MOP-4, Ubiquitin-activating enzyme E1-like protein 2, Ubiquitin-like modifier-activating enzyme 6)
Catalog No			USB-U1002
Supplier’s Catalog No			U1002
Supplier			US Biologicals
Source antigen			Recombinant, Sf9 cell
Reactivity
Cross reactivity
Applications
Molecular weight			118

Storage			-70°C
Other names
Grade			Purified
Purity			Purified ≥65%
Form			Supplied as a liquid in 40mM Tris, pH 8.0, 110mM sodium chloride, 2.2mM potassium chloride, 80ug/ml FLAG peptide, 3mM DTT, 20% glycerol.
Reactivity life			6 months
Note			For reserch purpose only
Purity			Purified ≥65%
Description			Recombinant corresponding to aa2-end of human UBA6 (UBE1L2) with N-terminal Flag tag expressed in a Baculovirus infected Sf9 cell expression system (NM_018227). Applications: Suitable for use in conjunction with E2 and E3 for the study of enzyme kinetics, screening inhibitors and selectivity profiling. Other applications not tested. Recommended Dilutions: Assay Conditions: UBA6 (100ng), UBCH7 (2800ng) and Flag-NEDD4 (800ng) were incubated in a 50ul reaction mix containing 50mM Tris, pH 7.4, 4mM ATP, 2mM DTT, 5mM magnesium chloride and 0.5ug biotin-Ub at 37°C for 30 minutes, followed by incubations with anti-FLAG antibody, anti-mouse HRP and reagents for chemiluminescence detection. Optimal dilutions to be determined by the researcher. Storage and Stability: Aliquot to avoid repeated freezing and thawing and store at -70°C. Aliquots are stable for 6 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.