Imugex

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Categories: Peptides & Proteins / Proteins

Product name			UBE2D2, Recombinant, Human, aa1-147, His-Tag (Ubiquitin-conjugating enzyme E2 D2, E2(17)KB2, PUBC1, UBC4, UBC4/5, UBCH5B)
Catalog No			USB-U1000-86P
Supplier’s Catalog No			U1000-86P
Supplier			US Biologicals
Source antigen			Recombinant, E.coli
Reactivity
Cross reactivity
Applications
Molecular weight			18.9

Storage			-20°C
Other names
Grade			Highly Purified
Purity			~95% (SDS-PAGE)
Form			Supplied as a liquid in 20mM Tris-HCl buffer, pH 8, 1mM DTT, 0.1M sodium chloride, 10% glycerol.
Reactivity life			6 months
Note			For reserch purpose only
Purity			~95% (SDS-PAGE)
Description			UBE2D2 is a member of the E2 ubiquitin-conjugating enzyme family. This enzyme functions in the ubiquitination of the tumor-suppressor protein p53, which is induced by an E3 ubiquitin-protein ligase. UBE2D2 catalyzes ubiquitination of IkB-alpha in a phosphorylation and SCFB-TRCP dependent manner. Purified by using conventional chromatography techniques. Source: Recombinant corresponding to aa1-147 of human UBE2D2, fused to His-tag at N-terminal, expressed in E.coli. AA Sequence: MGSSHHHHHH SSGLVPRGSH MALKRIHKEL NDLARDPPAQ CSAGPVGDDM FHWQATIMGP NDSPYQGGVF FLTIHFPTDY PFKPPKVAFT TRIYHPNINS NGSICLDILR SQWSPALTIS KVLLSICSLL CDPNPDDPLV PEIARIYKTD REKYNRIARE WTQKYAM Molecular Weight: ~18.9kD (167aa), confirmed by MALDI-TOF Enzyme Activity: Not determined. This product is recommended for use in applications that do not require a catalytically active form of the protein. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.