Imugex

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Categories: Peptides & Proteins / Proteins

Product name			UBE2W, Recombinant, Human, aa1-151, His-Tag (robable ubiquitin-conjugating enzyme E2 W, FLJ11011, hUBC-16)
Catalog No			USB-U1003-05
Supplier’s Catalog No			U1003-05
Supplier			US Biologicals
Source antigen			Recombinant, E.coli
Reactivity
Cross reactivity
Applications
Molecular weight			19.5

Storage			-20°C
Other names
Grade			Highly Purified
Purity			~90% (SDS-PAGE)
Form			Supplied as a liquid in 20mM Tris-HCl buffer, pH 8, 2mM DTT, 0.1M sodium chloride, 10% glycerol.
Reactivity life			6 months
Note			For reserch purpose only
Purity			~90% (SDS-PAGE)
Description			UBE2W accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. This protein in vitro catalyzes monoubiquitination and 'Lys-11'-linked polyubiquitination. UBE2W is widely expressed, especially at highest levels in testis. Purified by using conventional chromatography techniques. Source: Recombinant corresponding to aa1-151 of human UBE2W, fused to His-tag at N-terminus, expressed in E.coli. AA Sequence: MGSSHHHHHH SSGLVPRGSH MASMQKRLQK ELLALQNDPP PGMTLNEKSV QNSITQWIVD MEGAPGTLYE GEKFQLLFKF SSRYPFDSPQ VMFTGENIPV HPHVYSNGHI CLSILTEDWS PALSVQSVCL SIISMLSSCK EKRRPPDNSF YVRTCNKNPK KTKWWYHDDT C Molecular Weight: ~19.5kD (171aa), confirmed by MALDI-TOF Enzyme Activity: Not determined. This product is recommended for use in applications that do not require a catalytically active form of the protein. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.