Imugex

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Categories: Peptides & Proteins / Proteins

Product name			Ubiquitin-conjugating Enzyme E2 D2, Recombinant, Human, His-Tag (UBE2D2, UBC4, UBC5B, UBCH4, UBCH5B, Ubiquitin Carrier Protein D2, Ubiquitin-conjugating Enzyme E2(17)KB 2, Ubiquitin-conjugating Enzyme E2-17kD 2, Ubiquitin-protein Ligase D2)
Catalog No			USB-U1000-86Q
Supplier’s Catalog No			U1000-86Q
Supplier			US Biologicals
Source antigen			Recombinant, E. coli
Reactivity
Cross reactivity
Applications
Molecular weight			18

Storage			-70°C
Other names
Grade			Highly Purified
Purity			≥95% (SDS-PAGE)
Form			Supplied as a liquid in 20mM Tris-HCl, pH 7.5, 0.5mM DTT.
Reactivity life			6 months
Note			For reserch purpose only
Purity			≥95% (SDS-PAGE)
Description			UbcH5b is highly homologous to the S. cerevisiae UbC4/5 family of E2 enzymes, demonstrated to be essential for degradation of regulatory and abnormal proteins. UbcH5b, together with UbcH5a and UbcH5c, are the most active class of E2 enzymes in cell extracts and are associated with regulation of a number of transcription factors including p53. Useful for in vitro ubiquitinylation reactions.The His-tagged version of this enzyme is not susceptible to self-ubiquitinylation, which can occur with GST-tagged versions. Typical enzyme concentration to support in vitro conjugation is 100nM to 1uM depending upon the conditions. Recombinant corresponding to human UbcH5b, fused to His-tag, expressed in E. coli. Molecular Weight: ~18kD Storage and Stability: Aliquot to avoid repeated freezing and thawing and store at -70°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing and store at -70°C. Aliquots are stable for 6 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.