Imugex

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Categories: Peptides & Proteins / Proteins

Product name			Ubiquitin Aldehyde, Recombinant, Human (rh-Ubiquitin Aldehyde)
Catalog No			USB-U1000-06V
Supplier’s Catalog No			U1000-06V
Supplier			US Biologicals
Source antigen			Recombinant, E. coli
Reactivity
Cross reactivity
Applications
Molecular weight			8.5

Storage			4°C Do Not Freeze
Other names
Grade			Highly Purified
Purity			~95% (HPLC, SDS-PAGE).
Form			Supplied as a liquid in an aqueous solution, 0.15M hydrochloride.
Reactivity life			6 months
Note			For reserch purpose only
Purity			~95% (HPLC, SDS-PAGE).
Description			The C-terminal glycine carboxyl of Ubiquitin is synthetically modified to an aldehyde. Ubiquitin Aldehyde (Ub-H) is useful in the stabilization of ubiquitin-protein conjugates in vitro, enhancing their accumulation in cell lysates and tissue extracts. Inhibition of deubiquitinylating enzyme activity by Ub-H can be used to identify and confirm such activity and to determine the inhibition kinetics for a particular enzyme. Recommended concentration for maximal inhibition is 2-5uM. Co-crystallization of ubiquitin aldehyde with specific deubiquitinylating enzymes (the inhibitor mimics the natural ubiquitin substrate) has also been used to probe enzyme: substrate interactions. Source: Recombinant corresponding to human Ubiquitin Aldehyde expressed in E. coli. Molecular Weight: ~8.5kD Biological Activity: Ki = 2.5nM vs UCH isopeptidase-T Storage and Stability: May be stored at 4°C. For long-term storage, aliquot and store at 4°C. Do not freeze. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made in assay buffer.