Ubiquitin, Recombinant, Human

Catalog No : USB-U1000-06F
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Product name Ubiquitin, Recombinant, Human
Catalog No USB-U1000-06F
Supplier’s Catalog No U1000-06F
Supplier US Biologicals
Source antigen Recombinant, E.coli
Reactivity
Cross reactivity
Applications
Molecular weight 35
Storage -20°C
Other names
Grade Highly Purified
Purity ~95% (SDS-PAGE)
Form Supplied as a lyophilized powder from 20mM HEPES, pH 7.4. Reconstitute with sterile dH2O PBS.
Reactivity life 6 months
Note For reserch purpose only
Purity ~95% (SDS-PAGE)
Description Ubiquitin (Ub) is a small protein that is composed of 76aa. Ub is found only in eukaryotic organisms and is highly conserved. Ub can exist either in free form or as part of a complex with other proteins. In the latter case, Ub is attached (conjugated) to proteins through a covalent bond between the glycine at the C-terminal end of Ub and the side chains of lysine on the proteins; Ubiquitination. Functions to regulate protein turnover in a cell by closely regulating the degradation of specific proteins. Ubiquitin has been immunohistochemically localized to a number of pathological inclusions, including ; Lewy bodies of Parkinson's disease,neurofibrillary of Alzheimer's disease, Pick bodies of Pick's disease. Source: Recombinant corresponding to aa1-76, with N-GST tag, from human Ubiquitin, expressed in E.coli. Molecular Weight: ~35kD Storage and Stability: Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.