USP10, Active, Recombinant, Human, FLAG-Tag (Ubiquitin Carboxyl-terminal Hydrolase 10, Deubiquitinating Enzyme 10, Ubiquitin Thiolesterase 10, Ubiquitin-specific-processing Protease 10, KIAA0190)
Catalog No : USB-137394
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| Product name | USP10, Active, Recombinant, Human, FLAG-Tag (Ubiquitin Carboxyl-terminal Hydrolase 10, Deubiquitinating Enzyme 10, Ubiquitin Thiolesterase 10, Ubiquitin-specific-processing Protease 10, KIAA0190) | ||
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| Catalog No | USB-137394 | ||
| Supplier’s Catalog No | 137394 | ||
| Supplier | US Biologicals | ||
| Source antigen | Recombinant, Sf9 cell | ||
| Reactivity | |||
| Cross reactivity | |||
| Applications | |||
| Molecular weight | 88 | ||
| Storage | -70°C | ||
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| Other names | |||
| Grade | Purified | ||
| Purity | Purified (~60%) | ||
| Form | Supplied as a liquid in 45mM Tris-HCl, pH 8.0, 124mM sodium chloride, 2.4mM potassium chloride, 90ug/ml FLAG peptide, 10% glycerol, 3mM DTT. | ||
| Reactivity life | 6 months | ||
| Note | For reserch purpose only | ||
| Purity | Purified (~60%) | ||
| Description | USP10 is a hydrolase that can remove conjugated ubiquitin from target proteins such as p53/TP53, BECN1, SNX3 and CFTR. It acts as an essential regulator of p53/TP53 stability: in unstressed cells, specifically deubiquitinates p53/TP53 in the cytoplasm, leading to counteract MDM2 action and stabilize p53/TP53. Following DNA damage, USP10 translocates to the nucleus and deubiquitinates p53/TP53, leading to regulate the p53/TP53-dependent DNA damage response. It is a component of a regulatory loop that controls autophagy and p53/TP53 levels: mediates deubiquitination of BECN1, a key regulator of autophagy, leading to stabilize the PIK3C3/VPS34-containing complexes. In turn, PIK3C3/VPS34-containing complexes regulate USP10 stability, suggesting the existence of a regulatory system by which PIK3C3/VPS34-containing complexes regulate p53/TP53 protein levels via USP10 and USP13. USP10 does not deubiquitinate MDM2. It deubiquitinates CFTR in early endosomes, enhancing its endocytic recycling. Source: Recombinant corresponding to aa2-798 from human USP10, fused to FLAG-tag at N-terminal, expressedin Sf9 insect cells via a baculovirus expression system. Molecular Weight: ~88kD Applications: Suitable for for the study of enzyme kinetics, screening inhibitors, and selectivity profiling. Other applications not tested. Recommended Dilution: Optimal dilutions to be determined by the researcher. Storage and Stability: Aliquot to avoid repeated freezing and thawing and store at -70°C. Aliquots are stable for 6 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. | ||
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