JOSD1, Recombinant, Human, aa1-202, His-Tag (Josephin-1, dJ508I15.2)

Catalog No : USB-138674
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Product name JOSD1, Recombinant, Human, aa1-202, His-Tag (Josephin-1, dJ508I15.2)
Catalog No USB-138674
Supplier’s Catalog No 138674
Supplier US Biologicals
Source antigen Recombinant, E. coli
Reactivity
Cross reactivity
Applications
Molecular weight 25.6
Storage -20°C
Other names
Grade Highly Purified
Purity ~90% (SDS-PAGE)
Form Supplied as a liquid in 20mM tris-HCl buffer, pH 8.0, 0.2M sodium chloride, 5mM DTT, 2mM EDTA, 40% glycerol.
Reactivity life 6 months
Note For reserch purpose only
Purity ~90% (SDS-PAGE)
Description JOSD1, also as known as Josephin-1, has low protease activity towards poly-ubiquitin chains (in vitro). JOSD1 is act as a deubiquitinating enzyme. Recently identified ubiquitin-binding sites in the Josephin domain contribute to ubiquitin chain binding and cleavage. Source: Recombinant protein corresponding to aa1-202 from human JOSD1 at N-terminal, fused to His-tag expressed in E.coli. Molecular Weight: ~25.6kD (225aa) AA Sequence: MGSSHHHHHH SSGLVPRGSH MGS MVTPHKKSML GNGNYDVNVI MAALQTKGYE AVWWDKRRDV GVIALTNVMG FIMNLPSSLC WGPLKLPLKR QHWICVREVG GAYYNLDSKL MSCVPWK GDKAKSESLE LPQAAPPQIY HEKQRRELCA LHALNNVFQD SNAFTRDTLQ EIFQRLSPNT KMPEWIGGES ELRKFLKHHL RGKNCELLLV VPEEVEAHQS WRTDV Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.