Imugex

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Categories: Peptides & Proteins / Proteins

Product name			Ubiquitin Carboxyl Terminal Hydrolase L5, Recombinant, Human, aa1-329, His-Tag (UCHL5, Ubiquitin carboxyl-terminal hydrolase isozyme L5, UCH37)
Catalog No			USB-170094
Supplier’s Catalog No			170094
Supplier			US Biologicals
Source antigen			Recombinant, E. coli
Reactivity
Cross reactivity
Applications
Molecular weight			39.7

Storage			-20°C
Other names
Grade			Purified
Purity			~90% (SDS-PAGE)
Form			Supplied as a liquid in 20mM Tris-HCl, pH 8.0, 5mM DTT, 200mM NaCl, 0.1mM PMSF, 2mM EDTA, 30% glycerol.
Reactivity life			12 months
Note			For reserch purpose only
Purity			~90% (SDS-PAGE)
Description			UCHL5 is a member of the peptidase C12 family. It is a protease that specifically cleaves 'Lys-48'-linked polyubiquitin chains, and is the deubiquitinating enzyme related to the 19S regulatory subunit of the 26S proteasome. Source: Recombinant protein corresponding to aa1-329 from human UCHL5, fused to His-tag at N-terminal, expressed in E. coli. Molecular Weight: ~39.7kD (349aa, aa1-329 + NT His Tag) Biological Activity: Specific activity: >500pmole/min/ug. Measured by the hydrolysis of Ubiquitin-AMC at pH 8.0, at 37°C. AA Sequence: MGSSHHHHHH SSGLVPRGSH MTGNAGEWCL MESDPGVFTE LIKGFGCRGA QVEEIWSLEP ENFEKLKPVH GLIFLFKWQP GEEPAGSVVQ DSRLDTIFFA KQVINNACAT QAIVSVLLNC THQDVHLGET LSEFKEFSQS FDAAMKGLAL SNSDVIRQVH NSFARQQMFE FDTKTSAKEE DAFHFVSYVP VNGRLYELDG LREGPIDLGA CNQDDWISAV RPVIEKRIQK YSEGEIRFNL MAIVSDRKMI YEQKIAELQR QLAEEEPMDT DQGNSMLSAI QSEVAKNQML IEEEVQKLKR YKIENIRRKH NYLPFIMELL KTLAEHQQLI PLVEKAKEKQ NAKKAQETK Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.