Ubc12, Recombinant, Human (Ubiquitin Conjugating Enzyme 12)
Catalog No : USB-209286
473.58€
0.00€
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| Product name | Ubc12, Recombinant, Human (Ubiquitin Conjugating Enzyme 12) | ||
|---|---|---|---|
| Catalog No | USB-209286 | ||
| Supplier’s Catalog No | 209286 | ||
| Supplier | US Biologicals | ||
| Source antigen | Recombinant, E. coli | ||
| Reactivity | |||
| Cross reactivity | |||
| Applications | |||
| Molecular weight | 25 | ||
| Storage | -20°C | ||
|---|---|---|---|
| Other names | |||
| Grade | Highly Purified | ||
| Purity | ~95% (RP-HPLC, SDS-PAGE) | ||
| Form | Supplied as a lyophilized powder in PBS, pH 7.5, 1mM DTT. Reconstitute with 100ug/ml sterile ddH2O. | ||
| Reactivity life | 12 months | ||
| Note | For reserch purpose only | ||
| Purity | ~95% (RP-HPLC, SDS-PAGE) | ||
| Description | UbcH12 is functional in in vitro NEDDylation reactions. It has been shown to form a thioester linkage with NEDD8 in the presence of the NEDD8 activating enzyme complex Uba3/APP-BP1. APP-BP1 binds to the amyloid precursor protein (APP) carboxy terminal domain and is important in conjunction with Uba3 and UbcH12 in driving cells through the S to M checkpoint. It was demonstrated to be the E2 responsible for the NEDDylation of the Cul-1 component of the SCF (beta-TRCP) complex which is important as the E3-ligase in the ubiquitinylation of IkBa. NEDDylation of Cul-1 is essential for conjugation and processing of NF-kB p105 by SCF (beta-TRCP) following phosphorylation of the complex. A dominant negative form of UbcH12, previously demonstrated to sequester NEDD8 and inhibit its conjugation, inhibits both conjugation and processing of p105, which is alleviated by wild-type UbcH12. Source: Recombinant protein corresponding to 216aa from human Ubc12, fused to 6x His-tag, expressed in E. coli. Molecular Weight: ~25kD Storage and Stability: Lyophilized powder may be stored at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. | ||
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