Ubc9, Recombinant, Human (Ubiquitin Conjugating Enzyme 9)

Catalog No : USB-209288
473.58€
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Product name Ubc9, Recombinant, Human (Ubiquitin Conjugating Enzyme 9)
Catalog No USB-209288
Supplier’s Catalog No 209288
Supplier US Biologicals
Source antigen Recombinant, E. coli
Reactivity
Cross reactivity
Applications
Molecular weight 17.9
Storage -20°C
Other names
Grade Highly Purified
Purity ~95% (RP-HPLC, SDS-PAGE)
Form Supplied as a liquid in 50mM HEPES, pH 7.5, 150mM sodium chloride, 1mM DTT, 10% glycerol.
Reactivity life 12 months
Note For reserch purpose only
Purity ~95% (RP-HPLC, SDS-PAGE)
Description Human Ubc9 is homologous to ubiquitin-conjugating enzymes (E2s). However, instead of conjugating ubiquitin, it conjugates a ubiquitin homologue, small ubiquitin-like modifier 1(SUMO-1). And hUbc9 retains striking structural and functional conservation with yeast Ubc9. The ubiquitin-dependent protein degradation system has been recognized as a complete enzymatic pathway that is responsible for the selective degradation of abnormal and short-lived proteins. The conjugation of ubiquitin requires the activities of ubiquitin-activating (E1) and conjugating (E2) enzymes. Source: Recombinant protein corresponding to human Ubc9, a single, non-glycosylated polypeptide chain containing 154aa, expressed in E. coli. Molecular Weight: ~17.9kD Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.