Imugex

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Categories: Peptides & Proteins / Proteins

Product name			Ubiquitin Conjugating Enzyme 9, Recombinant, Human, His-Tag (Ubc9)
Catalog No			USB-209295
Supplier’s Catalog No			209295
Supplier			US Biologicals
Source antigen			Recombinant, E. coli
Reactivity
Cross reactivity
Applications
Molecular weight			19.5

Storage			-20°C
Other names
Grade			Highly Purified
Purity			~95% (RP-HPLC, SDS-PAGE)
Form			Supplied as a lyophilized powder in PBS, pH 7.5, 1mM DTT. Reconstitute with sterile ddH2O >100ug/ml. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Reactivity life			12 months
Note			For reserch purpose only
Purity			~95% (RP-HPLC, SDS-PAGE)
Description			Human Ubquitin Conjugating Enzyme 9 (Ubc9) is a member of the E2 family and is specific for the conjugation of SUMO to a variety of target proteins. SUMO conjugation to target proteins is mediated by a different, but analogous, pathway to ubiquitinylation. This E2 is unusual in that it interacts directly with protein substrates that are modified by sumolyation, and may play a role in substrate recognition. Ubc9 can mediate the conjugation of SUMO-1 to a variety of proteins including RanGAP1, IkBa, and PML without the requirement of an E3 ligase. Source: Recombinant protein corresponding to 171aa from human Ubc9, contains 6x His-tag, expressed in E. coli. Molecular Weight: ~19.5kD Storage and Stability: Lyophilized powder may be stored at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.