Ubiquitin, Recombinant, Human, His-Tag
Catalog No : USB-209297
508.06€
0.00€
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| Product name | Ubiquitin, Recombinant, Human, His-Tag | ||
|---|---|---|---|
| Catalog No | USB-209297 | ||
| Supplier’s Catalog No | 209297 | ||
| Supplier | US Biologicals | ||
| Source antigen | Recombinant, E. coli | ||
| Reactivity | |||
| Cross reactivity | |||
| Applications | |||
| Molecular weight | |||
| Storage | -20°C | ||
|---|---|---|---|
| Other names | |||
| Grade | Highly Purified | ||
| Purity | Highly Purified (~95%) | ||
| Form | Supplied as a liquid in PBS. | ||
| Reactivity life | 12 months | ||
| Note | For reserch purpose only | ||
| Purity | Highly Purified (~95%) | ||
| Description | Ubiquitin, a small protein consisting of 76aa, has been found in all eukaryotic cells studied. It is one of the most conserved proteins known. Two classes of ubiquitin genes are recognized. Class I is a polyubiquitin gene encoding a polyprotein of tandemly repeated ubiquitins. The class II genes are fusion products between a single ubiquitin gene and 1 of 2 other possible sequences, either 52 or 76 to 80 predominantly basic amino acids. Ubiquitin is required for ATP-dependent, nonlysosomal intracellular protein degradation, which eliminates most intracellular defective problems as well as normal proteins with a rapid turnover. Degradation involves covalent binding of ubiquitin to the protein to be degraded, through isopeptide bonds from the C-terminal glycine residue to the epsilon-amino groups of lysyl side chains. Presumably, the function of ubiquitin is to label the protein for disposal by intracellular proteases. The most abundant ubiquitin- protein conjugate, however, is ubiquitin-H2A, in which ubiquitin is bound to lys119 in histone H2A; this conjugate is not degraded. Since such ubiquitinated histones are present primarily in actively transcribed chromosomal regions, ubiquitin may play a role in regulation of gene expression. Source: Recombinant protein corresponding to human Ubiquitin, N-terminal his-taggged, expressed in E. coli. Applications: Suitable for use in ELISA, Western Blot and can be used for metal chelate affinity chromatography of ubiquinated molecules. It can also serve as a Positive Control for his-tagged antibodies. Other applications not tested. Recommended Dilution: ELISA: can be used to coat plates at 1ug/ml and detect with antibodies Western Blot: 50-200ng/lane Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. | ||
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