Mms2, Recombinant, Yeast, His-Tag (Ubiquitin-conjugating Enzyme E2 Variant 2, UEV-2, UBE2V2)
Catalog No : USB-220077
556.34€
0.00€
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| Product name | Mms2, Recombinant, Yeast, His-Tag (Ubiquitin-conjugating Enzyme E2 Variant 2, UEV-2, UBE2V2) | ||
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| Catalog No | USB-220077 | ||
| Supplier’s Catalog No | 220077 | ||
| Supplier | US Biologicals | ||
| Source antigen | Recombinant, Saccharomyces cerevisiae | ||
| Reactivity | |||
| Cross reactivity | |||
| Applications | |||
| Molecular weight | 15.54 | ||
| Storage | -70°C | ||
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| Other names | |||
| Grade | Highly Purified | ||
| Purity | ~95% (RP-HPLC) | ||
| Form | Supplied as a liquid in 50mM Tris, pH 7.5, 150mM sodium chloride, 10mM DTT, 10% glycerol. | ||
| Reactivity life | 12 months | ||
| Note | For reserch purpose only | ||
| Purity | ~95% (RP-HPLC) | ||
| Description | Mms2 is a UEV (ubiquitin E2 variant) protein that acts with the E2 enzyme Ubc13 in a heterodimeric complex which assembles K63-linked polyubiquitin chains utilized in non-degradative ubiquitin signaling pathways (DNA repair, signal transduction). The UEV proteins are structurally similar to E2 enzymes displaying the same fold, but lacking the active site cysteine of the E2. The Ubc13/Mms2 complex is unique in that it is has the ability to form free K63-linked polyubiquitin chains in solution in the absence of an E3. Source: Recombinant protein corresponding to yeast Mms2, fused to His-tag, expressed in Saccharomyces cerevisiae. Molecular Weight: ~15.54kD with His Tag. Biological Activity: Protein concentration of 100nM-1uM is recommended for in vitro conjugation. Storage and Stability: Aliquot to avoid repeated freezing and thawing and store at -70°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. | ||
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