Imugex

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Categories: Peptides & Proteins / Proteins

Product name			Ubiquitin Activating Enzyme E1, Recombinant, Human (UBE1, UBA1, POC20, A1S9T, A1ST, AMCX1, GXP1, SMAX2)
Catalog No			USB-220383
Supplier’s Catalog No			220383
Supplier			US Biologicals
Source antigen			Recombinant
Reactivity
Cross reactivity
Applications
Molecular weight			118

Storage			-70°C
Other names
Grade			Highly Purified
Purity			~95% (SDS-PAGE)
Form			Supplied as a liquid in 50mM Tris, pH 8.0, 100mM sodium chloride, 10% glycerol.
Reactivity life			12 months
Note			For reserch purpose only
Purity			~95% (SDS-PAGE)
Description			UBE1, the ubiquitin activating enzyme, is the first enzyme utilized in the pathway to conjugate ubiquitin onto target substrates through an ubiquitin-protein isopeptide bond. The C-terminal glycine of an ubiquitin (G76) is initially adenylated in an ATP dependent manner activating it so it can be transferred to the active site cysteine of UBE1 forming an ubiquitin-UBE1 thiolester. With the help of the other enzymes in the ubiquitin conjugation pathway (E2/E3 enzymes) the ubiquitin is transferred to a specific lysine on a target substrate. Source: Recombinant protein corresponding to human Ubiquitin Activating Enzyme E1. Molecular Weight: ~118kD Biological Activity: Typical enzyme concentration of 5nM-100nM is used for in vitro conjugation, depending on conditions. Storage and Stability: Aliquot to avoid repeated freezing and thawing and store at -70°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.