UBE2H, Recombinant, Human, aa1-183, His-Tag (Ubiquitin-conjugating enzyme E2H)

Catalog No : USB-350368
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Product name UBE2H, Recombinant, Human, aa1-183, His-Tag (Ubiquitin-conjugating enzyme E2H)
Catalog No USB-350368
Supplier’s Catalog No 350368
Supplier US Biologicals
Source antigen Recombinant, E. coli
Reactivity
Cross reactivity
Applications
Molecular weight 23.1
Storage -20°C
Other names
Grade Highly Purified
Purity ~95% (SDS-PAGE)
Form Supplied as a liquid in 20mM Tris-HCl, pH 8.0, 1mM DTT, 50mM sodium chloride, 10% glycerol.
Reactivity life 6 months
Note For reserch purpose only
Purity ~95% (SDS-PAGE)
Description uBE2H (ubiquitin-conjugating enzyme E2 H) belongs to the ubiquitin-conjugating enzyme family. The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. uBE2H accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. Source: Recombinant protein corresponding to aa1-183 of human uBE2H, fused to His-tag at N-terminal expressed in E. coli. Molecular Weight: ~23.1KD AA Sequence: MGSSHHHHHH SSGLVPRGSH MGSMSSPSPG KRRMDTDVVK LIESKHEVTI LGGLNEFVVK FYGPQGTPYE GGVWKVRVDL PDKYPFKSPS IGFMNKIFHP NIDEASGTVC LDVINQTWTA LYDLTNIFES FLPQLLAYPN PIDPLNGDAA AMYLHRPEEY KQKIKEYIQK YATEEALKEQ EEGTGDSSSE SSMSDFSEDE AQDMEL Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 6 months after receipt at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.