uFSP1, Recombinant, Human, aa1-142, His-Tag (Inactive Ufm1-specific protease 1, UFSP)

Catalog No : USB-351563
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Product name uFSP1, Recombinant, Human, aa1-142, His-Tag (Inactive Ufm1-specific protease 1, UFSP)
Catalog No USB-351563
Supplier’s Catalog No 351563
Supplier US Biologicals
Source antigen Recombinant, human from E. coli
Reactivity
Cross reactivity
Applications
Molecular weight 17
Storage -20°C/-70°C
Other names
Grade Purified
Purity ~90% (SDS-PAGE)
Form Supplied as a liquid in 20mM Tris-HCl, pH 8.5, 0.2M sodium chloride, 1mM DTT, 30% glycerol.
Reactivity life 12 months
Note For reserch purpose only
Purity ~90% (SDS-PAGE)
Description UFSP1 is similar to other Ufm1-specific proteases. Studies in mouse determined that Ufsp1 releases Ufm1 (ubiquitin-fold modifier 1) from its bound conjugated complexes which also makes it into an active form. Because the human UFSP1 protein is shorter on the N-terminal and lacks a conserved Cys active site, it is predicted to be non-functional. Source: Recombinant protein corresponding to aa1-142 from human UFSP1, fused to His-tag at N-terminal expressed in E. coli. Molecular Weight: ~17kD(165aa) AA Sequence: MGSSHHHHHH SSGLVPRGSH MGSMGDKPPG FRGSRDWIGC VEASLCLAHF GGPQGRLCHV PRGVGLHGEL ERLYSHFAGG GGPVMVGGDA DARSKALLGV CVGSGTEAYV LVLDPHYWGT PKSPSELQAA GWVGWQEVSA AFDPNSFYNL CLTSLSSQQQ QRTLD Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.