Imugex

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Categories: Peptides & Proteins / Proteins

Product name			Uchl1, Recombinant, Mouse, aa1-223, His-Tag (Ubiquitin Carboxyl-terminal Hydrolase Isozyme L1)
Catalog No			USB-377997
Supplier’s Catalog No			377997
Supplier			US Biologicals
Source antigen			Recombinant, E. coli
Reactivity
Cross reactivity
Applications
Molecular weight			27.2

Storage			-20°C
Other names
Grade			Highly Purified
Purity			~90% (SDS-PAGE)
Form			Supplied as a liquid in PBS, 1mM DTT, 10% glycerol.
Reactivity life			6 months
Note			For reserch purpose only
Purity			~90% (SDS-PAGE)
Description			Uchl1 also known as Ubiquitin carboxyl-terminal hydrolase isozyme L1 is a member of a gene family whose products hydrolyze small C-terminal adducts of ubiquitin to generate the ubiquitin monomer. UCHL1 is a component of the ubiquitin system, which has a fundamental role in regulating various biological activities. Source: Recombinant protein corresponding to aa24-188 from mouse Uchl1, fused to His-Tag at N-terminal, expressed in E. coli. Molecular Weight: ~27.2kD (246aa), confirmed by MALDI-TOF Biological Activity: Specific activity is >70pmol/min/ug, and is defined as the amount of enzyme that hydrolysis 1pmole of ubiquitin-AMC/minute at pH 7.5, at 37°C. AA Sequence: MGSSHHHHHH SSGLVPRGSH MGSMQLKPME INPEMLNKVL AKLGVAGQWR FADVLGLEEE TLGSVPSPAC ALLLLFPLTA QHENFRKKQI EELKGQEVSP KVYFMKQTIG NSCGTIGLIH AVANNQDKLE FEDGSVLKQF LSETEKLSPE DRAKCFEKNE AIQAAHDSVA QEGQCRVDDK VNFHFILFNN VDGHLYELDG RMPFPVNHGA SSEDSLLQDA AKVCREFTER EQGEVRFSAV ALCKAA Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.