Ubiquitin Conjugating Enzyme E2T, Recombinant, Human (Ubiquitin-conjugating Enzyme E2 T, UBE2T, Cell Proliferation-inducing Gene 50 Protein, HSPC150, PIG50, Ubiquitin Carrier Protein T, Ubiquitin-protein Ligase T)
Catalog No : USB-U1000-97
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| Product name | Ubiquitin Conjugating Enzyme E2T, Recombinant, Human (Ubiquitin-conjugating Enzyme E2 T, UBE2T, Cell Proliferation-inducing Gene 50 Protein, HSPC150, PIG50, Ubiquitin Carrier Protein T, Ubiquitin-protein Ligase T) | ||
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| Catalog No | USB-U1000-97 | ||
| Supplier’s Catalog No | U1000-97 | ||
| Supplier | US Biologicals | ||
| Source antigen | Recombinant, E. coli | ||
| Reactivity | |||
| Cross reactivity | |||
| Applications | |||
| Molecular weight | 23.6 | ||
| Storage | -20°C | ||
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| Other names | |||
| Grade | Highly Purified | ||
| Purity | ~ 95% (SDS-PAGE). Purified by using conventional chromatography techniques. | ||
| Form | Supplied as a liquid in 20mM Tris HCl, pH 8.0, 1mM DTT, 10% glycerol. | ||
| Reactivity life | 6 months | ||
| Note | For reserch purpose only | ||
| Purity | ~ 95% (SDS-PAGE). Purified by using conventional chromatography techniques. | ||
| Description | UBE2T, also known as PIG50 or HSPC150, is a member of the E2 ubiquitin-conjugating enzyme family Involved in the protein degradation pathway. This protein catalyzes the ATP-dependent attachment of ubiquitin (Ub) to target proteins, thereby tagging them for subsequent destruction by the proteasome. Additionally, UBE2T is thought to be a crucial component of the Faconi anemia pathway of DNA damage repair and, upon self-inactivation, may negatively regulate the Faconi pathway. Recombinant UBE2T protein, fused to His-tag at C-terminus, was expressed in E. coli. Source: Recombinant corresponding to aa1-197 of human UBE2T 6x His tagged expressed in E. coli. AA Sequence: MQRASRLKRE LHMLATEPPP GITCWQDKDQ MDDLRAQILG GANTPYEKGV FKLEVIIPER YPFEPPQIRF LTPIYHPNID SAGRICLDVL KLPPKGAWRP SLNIATVLTS IQLLMSEPNP DDPLMADISS EFKYNKPAFL KNARQWTEKH ARQKQKADEE EMLDNLPEAG DSRVHNSTQK RKASQLVGIE KKFHPDVLEH HHHHH Enzyme Activity: Not determined. This product is recommended for use in applications that do not require a catalytically active form of the protein. Applications: Suitable for use in Western Blot. Other applications not tested. Recommended Dilution: Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 6 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. | ||
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