Nedd 4, Recombinant, Human, N-terminal FLAG-Tag (Cell proliferation-inducing gene 53 protein, E3 ubiquitin-protein ligase NEDD4, KIAA0093, MGC176705, NEDD-4, NEDD4-1, Neural precursor cell expressed developmentally down-regulated protein 4, PIG53, RPF1)
Catalog No : USB-N0578
652.89€
0.00€
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| Product name | Nedd 4, Recombinant, Human, N-terminal FLAG-Tag (Cell proliferation-inducing gene 53 protein, E3 ubiquitin-protein ligase NEDD4, KIAA0093, MGC176705, NEDD-4, NEDD4-1, Neural precursor cell expressed developmentally down-regulated protein 4, PIG53, RPF1) | ||
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| Catalog No | USB-N0578 | ||
| Supplier’s Catalog No | N0578 | ||
| Supplier | US Biologicals | ||
| Source antigen | Recombinant, Sf9 cell | ||
| Reactivity | |||
| Cross reactivity | |||
| Applications | |||
| Molecular weight | 86 | ||
| Storage | -70°C | ||
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| Other names | |||
| Grade | Purified | ||
| Purity | ~50% (SDS-PAGE) | ||
| Form | Supplied as a liquid in 40mM Tris-HCl, pH 8.0, 110mM sodium chloride, 2.2mM potassium chloride, 80ug/ml FLAG peptide, 3mM DTT, 20% glycerol. | ||
| Reactivity life | 6 months | ||
| Note | For reserch purpose only | ||
| Purity | ~50% (SDS-PAGE) | ||
| Description | Source: Recombinant corresponding to aa150-end of human Nedd 4 with N-terminal FLAG tag, expressed in a Baculovirus infected Sf9 cell expression system (NM_006154). Specific Activity: 0.0027pmol/min/ug Assay conditions: 50ul reaction mix (4mM ATP, 5mM MgCl2, 1mM DTT, E1 (0.5ug UBE1), E2 (2.8ug UBCH5a), Biotin-Ubiquitin (0.5ug) and Nedd4 (0-2ug ) in 50mM Tris pH 7.4) incubate for 30 min at 37ºC, then add to streptavidin coated plates and incubate for 30 min at 37ºC. Add antibody against Nedd4, incubate 1 hour. Finally, incubate 1 hour with the Europeum-labeled secondary antibody. Time resolved fluorescence measured at RT. (lambda-exc=330nm, lambda-em=620nm). Applications: Suitable for use in conjunction with E1 and E2 for the study of enzyme kinetics, screening inhibitors and selectivity profiling. Other applications not tested. Recommended Dilution: Optimal dilutions to be determined by the researcher. Storage and Stability: Aliquot to avoid repeated freezing and thawing and store at -70°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquots are stable for 6 months. | ||
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