Smad Ubiquitination Regulatory Factor-1, N-terminal FLAG-Tag, Recombinant, Human (Smad Ubiquitination Regulatory Factor 1, Smurf1, E3 Ubiquitin Ligase SMURF1, HGNC:16807, KIAA1625, Smad-specific E3 Ubiquitin Ligase 1, SMAD-specific E3 Ubiquitin Protein Ligase 1)
Catalog No : USB-S1014-60R
640.25€
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| Product name | Smad Ubiquitination Regulatory Factor-1, N-terminal FLAG-Tag, Recombinant, Human (Smad Ubiquitination Regulatory Factor 1, Smurf1, E3 Ubiquitin Ligase SMURF1, HGNC:16807, KIAA1625, Smad-specific E3 Ubiquitin Ligase 1, SMAD-specific E3 Ubiquitin Protein Ligase 1) | ||
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| Catalog No | USB-S1014-60R | ||
| Supplier’s Catalog No | S1014-60R | ||
| Supplier | US Biologicals | ||
| Source antigen | Recombinant, Sf9 cell | ||
| Reactivity | |||
| Cross reactivity | |||
| Applications | |||
| Molecular weight | 70 | ||
| Storage | -70°C | ||
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| Other names | |||
| Grade | Purified | ||
| Purity | Purified ≥80% | ||
| Form | Supplied as a liquid in 25mM Tris-HCl, pH 8.0, 100mM sodium chloride, 0.05% Tween 20, 100ug/ml FLAG peptide, 3mM DTT, 20% glycerol. | ||
| Reactivity life | 6 months | ||
| Note | For reserch purpose only | ||
| Purity | Purified ≥80% | ||
| Description | Source: Recombinant corresponding to aa150-end of human SMURF 1, fused with N-terminal FLAG tag expressed in a Baculovirus infected Sf9 cell expression system (NM_020429). Specific Activity: 0.0055pmol/min/ug Assay conditions: 50ul reaction mix (4mM ATP, 5mM MgCl2, 1mM DTT, E1 (0.5ug), E2 (2.8ug), Biotin-Ubiquitin (0.5ug)and Smurf1 (0-1ug) in 50mM Tris pH 7.4) incubate for 30min at 37ºC, then add to streptavidin coated plates and incubate for 30min at 37ºC. Add antibody against Smurf1, incubate 1 hour. Finally, incubate 1 hour with the Europeum-labeled secondary antibody. Time resolved fluorescence measured at room temperature (lambda-exc=330 nm, lambda-em=620 nm). Applications: Suitable for use in conjunction with E1 and E2 for the study of enzyme kinetics, screening inhibitors and selectivity profiling. Other applications not tested. Recommended Dilution: Optimal dilutions to be determined by the researcher. Storage and Stability: Aliquot to avoid repeated freezing and thawing. Store at -70°C. Aliquots are stable for at least 6 months at -70°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. | ||
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