Imugex

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Categories: Peptides & Proteins / Proteins

Product name			UBE2B, aa1-152, Recombinant, Human (HHR6B, HR6B, RAD6B, RAD6 Homolog B, Ubiquitin Carrier Protein B, Ubiquitin-conjugating Enzyme E2-17kD, Ubiquitin-protein Ligase B)
Catalog No			USB-U0879-89
Supplier’s Catalog No			U0879-89
Supplier			US Biologicals
Source antigen			Recombinant, E.coli
Reactivity
Cross reactivity
Applications
Molecular weight			21.4

Storage			-20°C
Other names
Grade			Purified
Purity			~90% (SDS-PAGE).
Form			Supplied as a liquid in 20mM Tris-HCl, pH 8.0, 10% glycerol, 0.1M sodium chloride.
Reactivity life			6 months
Note			For reserch purpose only
Purity			~90% (SDS-PAGE).
Description			UBE2B, also known as ubiquitin-conjugating enzyme E2 B, belongs to E2 ubiquitin-conjugating enzyme family. This protein catalyzes the covalent attachment of ubiquitin to other proteins, and associates to the E3 ligase RAD18 to form the UBE2B-RAD18 ubiquitin ligase complex involved in mono-ubiquitination of DNA-associated PCNA on 'Lys-164'. It is required for postreplication repair of UV-damaged DNA. It may be involved in neurite outgrowth. Source: Recombinant corresponding to aa1-152 of human UBE2B 6x his tagged, expressed in E.coli. AA Sequences: MRGSHHHHHH GMASMTGGQQ MGRDLYDDDD KDRWGSMSTP ARRRLMRDFK RLQEDPPVGV SGAPSENNIM QWNAVIFGPE GTPFEDGTFK LVIEFSEEYP NKPPTVRFLS KMFHPNVYAD SICLDILQN RWSPTYDVSS ILTSIQSLLD EPNPNSPANS QAAQLYQENK REYEKRVSAI VEQSWNDS Enzyme Activity: Not determined. This product is recommended for use in applications that do not require a catalytically active form of the protein. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing.. Store at -20°C. Aliquots are stable for at least 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.