UBE2N, Recombinant, Human, aa1-152, His-Tag (Ubiquitin-conjugating Enzyme E2 N, Ubiquitin-protein Ligase N, Ubiquitin Carrier Protein N, Ubc13, Bendless-like Ubiquitin Conjugating Enzyme, BLU)
Catalog No : USB-U1000-92Z
508.06€
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| Product name | UBE2N, Recombinant, Human, aa1-152, His-Tag (Ubiquitin-conjugating Enzyme E2 N, Ubiquitin-protein Ligase N, Ubiquitin Carrier Protein N, Ubc13, Bendless-like Ubiquitin Conjugating Enzyme, BLU) | ||
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| Catalog No | USB-U1000-92Z | ||
| Supplier’s Catalog No | U1000-92Z | ||
| Supplier | US Biologicals | ||
| Source antigen | Recombinant, E. coli | ||
| Reactivity | |||
| Cross reactivity | |||
| Applications | |||
| Molecular weight | 19.3 | ||
| Storage | -20°C | ||
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| Other names | |||
| Grade | Highly Purified | ||
| Purity | ~95% (SDS-PAGE) | ||
| Form | Supplied as a liquid in 20mM Tris-HCl buffer, pH 8.0, 0.1M sodium chloride, 1mM DTT, 20% glycerol. | ||
| Reactivity life | 6 months | ||
| Note | For reserch purpose only | ||
| Purity | ~95% (SDS-PAGE) | ||
| Description | UBE2N, also known as UBC13, is a member of the E2 ubiquitin-conjugating enzyme family. It catalyzes the ATP-dependent synthesis of non-canonical polyubiquitin chains, a process that does not lead to proteasomal degradation. It mediates the transcription of several target genes and is thought to play a role in cell cycle progression; cellular differentiation and DNA repair mechanisms that ensure cell survival after DNA damage. Source: Recombinant corresponding to aa1-152 from human UBE2N, fused to His-tag at N-terminus expressed in E. coli. AA Sequence: MGSSHHHHHH SSGLVPRGSH MAGLPRRIIK ETQRLLAEPV PGIKAEPDES NARYFHVVIA GPQDSPFEGG TFKLELFLPE EYPMAAPKVR FMTKIYHPNV DKLGRICLDI LKDKWSPALQ IRTVLLSIQA LLSAPNPDDP LANDVAEQWK TNEAQAIETA RAWTRLYAMN NI Molecular Weight: ~19.3kD (172aa), confirmed by MALDI-TOF Enzyme Activity: Not determined. This product is recommended for use in applications that do not require a catalytically active form of the protein. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. | ||
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