Imugex

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Categories: Peptides & Proteins / Proteins

Product name			UbcH1, Recombinant, Human (Huntingtin-interacting Protein 2, HIP2, HIP-2, LIG, Ubiquitin Carrier Protein, Ubiquitin-conjugating Enzyme E2 K, UBE2K, Ubiquitin-conjugating Enzyme E2-25kD, Ubiquitin-conjugating Enzyme E2(25K), Ubiquitin-conjugating Enzyme E2-25K, Ubiquitin-protein Ligase)
Catalog No			USB-U0850-15A
Supplier’s Catalog No			U0850-15A
Supplier			US Biologicals
Source antigen			Recombinant, E. coli
Reactivity
Cross reactivity
Applications
Molecular weight			22.4

Storage			-70°C
Other names
Grade			Highly Purified
Purity			~95% (SDS-PAGE)
Form			Supplied as a liquid in 20mM tris buffered saline, pH 7.5, 1mM DTT.
Reactivity life			6 months
Note			For reserch purpose only
Purity			~95% (SDS-PAGE)
Description			Among ubiquitin-conjugating enzymes, the mammalian ubiquitin conjugating enzyme UbcH1, is unique in its ability to catalyze the in vitro synthesis of unanchored Lys48-linked poly-ubiquitin chains from mono- or poly-ubiquitin, E1, and ATP. In addition, UbcH1 can catalyze the cyclization of longer poly-ubiquitin chains, including tetra- and penta-ubiquitin. Recombinant UbcH1 charges and supports ubiquitinylation in vitro. Typical enzyme concentration to support conjugation in vitro is 100nM to 1uM. Source: Recombinant corresponding to human UbcH1 with GST-tag, expressed in E. coli. Molecular Weight: ~22.4kD Storage and Stability: Aliquot to avoid repeated freezing and thawing and store at -70°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.