Imugex

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Categories: Peptides & Proteins / Proteins

Product name			Ubiquitin-conjugating Enzyme E2 H, Recombinant, Human, His-Tag (UBE2H, UbcH2, Ubiquitin Carrier Protein H, Ubiquitin-conjugating Enzyme E2-20K, Ubiquitin-protein Ligase H)
Catalog No			USB-U1000-87A1
Supplier’s Catalog No			U1000-87A1
Supplier			US Biologicals
Source antigen			Recombinant, E. coli
Reactivity
Cross reactivity
Applications
Molecular weight			22.2

Storage			-70°C
Other names
Grade			Highly Purified
Purity			~95% (SDS-PAGE)
Form			Supplied as a liquid in 20mM Tris-HCl, pH 7.5, 0.5mM DTT.
Reactivity life			6 months
Note			For reserch purpose only
Purity			~95% (SDS-PAGE)
Description			UbcH2 is the human homolog to the yeast DNA repair gene RAD6, which is induced by DNA damaging reagents. It has been shown to conjugate ubiquitin to histone H2A in an E3 dependent manner in vitro. UbcH2 consists of two functionally independent domains, an N-terminal core domain with ubiquitin conjugating activity, and a C-terminal domain that interacts with substrate proteins. This protein is useful for in vitro ubiquitinylation reactions. The His-tagged version of this enzyme is not susceptible to self-ubiquitinylation, which can occur with GST-tagged versions. Typical enzyme concentration to support conjugation in vitro is 100nM to 1uM depending upon conditions. Source: Recombinant corresponding to human UbcH2 with His-tag, expressed in E. coli. Molecular Weight: ~22.2kD Storage and Stability: Aliquot to avoid repeated freezing and thawing and store at -70°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.