Imugex

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Categories: Peptides & Proteins / Proteins

Product name			Ubiquitin-conjugating Enzyme E2 D1, Recombinant, Human, His-Tag (UBE2D1, Stimulator of Fe Transport, SFT, UBC4/5 Homolog, UBC5A, UbcH5, UBCH5A, Ubiquitin Carrier Protein D1, Ubiquitin-conjugating Enzyme E2(17)KB 1, Ubiquitin-conjugating Enzyme E2-17kD 1, Ubiquitin-protein Ligase D1)
Catalog No			USB-U1000-86N1
Supplier’s Catalog No			U1000-86N1
Supplier			US Biologicals
Source antigen			Recombinant, E. coli
Reactivity
Cross reactivity
Applications
Molecular weight			17

Storage			-70°C
Other names
Grade			Highly Purified
Purity			~95% (SDS-PAGE)
Form			Supplied as a liquid in 20mM Tris-HCl, pH 7.5, 0.5mM DTT.
Reactivity life			6 months
Note			For reserch purpose only
Purity			~95% (SDS-PAGE)
Description			UbcH5a is highly homologous to the S. cerevisiae Ubc4/5 family of E2 enzymes demonstrated to be essential for degradation of regulatory and abnormal proteins. UbcH5a, together with UbcH5b and UbcH5c, are the most active class of E2 enzymes in cell extracts and are associated with regulation of a number of transcription factors. Specifically, UbcH5a stimulates the conjugation of ubiquitin to the tumor suppressor p532. Useful for in vitro ubiquitinylation reactions. Typical enzyme concentration to support conjugation in vitro is 100nM to 1uM depending upon conditions. Source: Recombinant corresponding to human UbcH5a with His-tag, expressed in E. coli. Molecular Weight: ~17kD Storage and Stability: Aliquot to avoid repeated freezing and thawing and store at -70°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.