UBE2D3, Recombinant, Human, aa1-149, His-Tag (Ubiquitin-conjugating Enzyme E2 D3, Ubiquitin-protein Ligase D3, Ubiquitin carrier protein D3, Ubiquitin-conjugating Enzyme E2-17kD 3, Ubiquitin-conjugating Enzyme E2(17)KB 3, UBCH5C)
Catalog No : USB-U0880-08D
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| Product name | UBE2D3, Recombinant, Human, aa1-149, His-Tag (Ubiquitin-conjugating Enzyme E2 D3, Ubiquitin-protein Ligase D3, Ubiquitin carrier protein D3, Ubiquitin-conjugating Enzyme E2-17kD 3, Ubiquitin-conjugating Enzyme E2(17)KB 3, UBCH5C) | ||
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| Catalog No | USB-U0880-08D | ||
| Supplier’s Catalog No | U0880-08D | ||
| Supplier | US Biologicals | ||
| Source antigen | Recombinant, E.coli | ||
| Reactivity | |||
| Cross reactivity | |||
| Applications | |||
| Molecular weight | 19.1 | ||
| Storage | -20°C | ||
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| Other names | |||
| Grade | Highly Purified | ||
| Purity | ~90% (SDS-PAGE) | ||
| Form | Supplied as a liquid in 20mM Tris-HCl buffer, pH 8.0, 40% glycerol, 0.15M sodium chloride, 1mM DTT. | ||
| Reactivity life | 6 months | ||
| Note | For reserch purpose only | ||
| Purity | ~90% (SDS-PAGE) | ||
| Description | UBE2D3, the modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This enzyme functions in the ubiquitination of the tumor-suppressor protein p53, which is induced by an E3 ubiquitin-protein ligase. Source: Recombinant corresponding to aa1-149 from human UBE2D3, fused to His-tag at N-terminal expressed in E.coli. AA Sequence: MGSSHHHHHH SSGLVPRGSH MLSNRKCLSK ELSDLARDPP AQCSAGPVGD DMFHWQATIM GPNDSPYQGG VFFLTIHFPT DYPFKPPKVA FTTRIYHPNI NSNGSICLDI LRSQWSPALT ISKVLLSICS LLCDPNPDDP LVPEIARIYK TDRDKYNRIS REWTQKYAM Molecular Weight: ~19.1kD (MALDI-TOF) Enzyme Activity: Not determined. This product is recommended for use in applications that do not require a catalytically active form of the protein. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. | ||
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