RNF181, Recombinant, Human, aa1-153, His-Tag (E3 Ubiquitin-protein Ligase RNF181, HSPC238)

Catalog No : USB-138729
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Product name RNF181, Recombinant, Human, aa1-153, His-Tag (E3 Ubiquitin-protein Ligase RNF181, HSPC238)
Catalog No USB-138729
Supplier’s Catalog No 138729
Supplier US Biologicals
Source antigen Recombinant, E. coli
Reactivity
Cross reactivity
Applications
Molecular weight 20.3
Storage -20°C
Other names
Grade Highly Purified
Purity ~95% (SDS-PAGE)
Form Supplied as a liquid in 20mM tris-HCl buffer, pH 8.0, 0.15M sodium chloride, 1mM DTT, 10% glycerol.
Reactivity life 6 months
Note For reserch purpose only
Purity ~95% (SDS-PAGE)
Description E3 ubiquitin-protein ligase RNF181, also known as RNF181, accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. Source: Recombinant protein corresponding to aa1-153 from human RNF181 at N-terminal, fused to His-tag expressed in E.coli. Molecular Weight: ~20.3kD (176aa) AA Sequence: MGSSHHHHHH SSGLVPRGSH MGS RGSQAELKCP VCLLEFEEEE TAIEMPCHHL FHSSCILPWL SKTNSCPLCR YELPTDDDTY EEHRRDKARK QQQQHRLENL HGAMYT MASYFDE HDCEPSDPEQ ETRTNMLLEL ARSLFNRMDF EDLGLVVDWD HHLPPPAAKT VVENLPRTVI Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.