HPV E6, Recombinant, Viral (HpV16gp1, HPV Early Protein 6, Papillomavirus E6)
Catalog No : USB-145802
455.19€
0.00€
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| Product name | HPV E6, Recombinant, Viral (HpV16gp1, HPV Early Protein 6, Papillomavirus E6) | ||
|---|---|---|---|
| Catalog No | USB-145802 | ||
| Supplier’s Catalog No | 145802 | ||
| Supplier | US Biologicals | ||
| Source antigen | Recombinant, E. coli | ||
| Reactivity | |||
| Cross reactivity | |||
| Applications | |||
| Molecular weight | 19 | ||
| Storage | -70°C | ||
|---|---|---|---|
| Other names | |||
| Grade | Highly Purified | ||
| Purity | ~98% (SDS-PAGE) under reducing conditions and visualized by Colloidal Coomassie Blue Stain. | ||
| Form | Supplied as a liquid in 50mM Hepes, pH 7.0, 400mM sodium chloride, 2mM DTT. BSA free. | ||
| Reactivity life | 6 months | ||
| Note | For reserch purpose only | ||
| Purity | ~98% (SDS-PAGE) under reducing conditions and visualized by Colloidal Coomassie Blue Stain. | ||
| Description | E6 (Early protein 6) is a viral protein produced in cells infected with the Human Papillomavirus. E6 forms a complex with the host cell ubiquitin ligase E6AP (E6-Associated-Protein) generating a ligase activity that polyubiquitinates tumor suppressors p53 and p73 and targets them to the 26S proteasome for degradation. As a result DNA damage and chromosomal instabilities increase, often leading to cell proliferation and cancer. The E6/E6AP complex also targets other substrates for ubiquitination, such as TERT, BAK1, FADD, and pro-CASP8—none of which appear to be substrates for E6AP in the absence of E6. This protein is untagged, and contains six cysteine-to-serine substitutions at positions 23, 58, 87, 104, 118, and 147 (HPV type 16 sequence numbering). Source: Recombinant protein corresponding to HPV E6, expressed in E. coli. Contains Cys to Ser substitutions at the following positions: 23, 58, 87, 104, 118 and 147. Activity: Typical concentration to support E6AP-mediated p53 ubiquitination in vitro is 0.1-2uM depending on experimental conditions. Storage and Stability: Aliquot to avoid repeated freezing and thawing and store at -70°C. Aliquots are stable for 6 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. | ||
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