UbcH1, Recombinant, Human, GST-tag (Ubiquitin Conjugating Enzyme 1, Ubiquitin Conjugating Enzyme)
Catalog No : USB-220370
432.20€
0.00€
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| Product name | UbcH1, Recombinant, Human, GST-tag (Ubiquitin Conjugating Enzyme 1, Ubiquitin Conjugating Enzyme) | ||
|---|---|---|---|
| Catalog No | USB-220370 | ||
| Supplier’s Catalog No | 220370 | ||
| Supplier | US Biologicals | ||
| Source antigen | Recombinant, E. coli | ||
| Reactivity | |||
| Cross reactivity | |||
| Applications | |||
| Molecular weight | 22.4 | ||
| Storage | -70°C | ||
|---|---|---|---|
| Other names | |||
| Grade | Highly Purified | ||
| Purity | ~95% (SDS-PAGE) | ||
| Form | Supplied as a liquid in 20mM TBS, pH 7.5, 1mM DTT. | ||
| Reactivity life | 12 months | ||
| Note | For reserch purpose only | ||
| Purity | ~95% (SDS-PAGE) | ||
| Description | Among ubiquitin-conjugating enzymes, the mammalian ubiquitin conjugating enzyme UbcH1, is unique in its ability to catalyze the in vitro synthesis of unanchored Lys48-linked poly-ubiquitin chains from mono- or poly-ubiquitin, E1, and ATP. In addition, UbcH1 can catalyze the cyclization of longer poly-ubiquitin chains, including tetra- and penta-ubiquitin. Recombinant UbcH1 charges and supports ubiquitinylation in vitro. Typical enzyme concentration to support conjugation in vitro is 100nM-1uM. Source: Recombinant protein corresponding to human UbcH1, fused to GST-tag, expressed in E. coli. Molecular Weight: ~22.4kD Storage and Stability: Aliquot to avoid repeated freezing and thawing and store at -70°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. | ||
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