UbcH5b, Recombinant, Human, His-Tag (UB2D2, Ubiquitin Conjugating Enzyme H5b, Ubiquitin Conjugating Enzyme)
Catalog No : USB-220372
432.20€
0.00€
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| Product name | UbcH5b, Recombinant, Human, His-Tag (UB2D2, Ubiquitin Conjugating Enzyme H5b, Ubiquitin Conjugating Enzyme) | ||
|---|---|---|---|
| Catalog No | USB-220372 | ||
| Supplier’s Catalog No | 220372 | ||
| Supplier | US Biologicals | ||
| Source antigen | Recombinant, E. coli | ||
| Reactivity | |||
| Cross reactivity | |||
| Applications | |||
| Molecular weight | 18 | ||
| Storage | -70°C | ||
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| Other names | |||
| Grade | Highly Purified | ||
| Purity | ~95% (SDS-PAGE) | ||
| Form | Supplied as a liquid in 20mM Tris-HCl, pH 7.5, 0.5mM DTT. | ||
| Reactivity life | 12 months | ||
| Note | For reserch purpose only | ||
| Purity | ~95% (SDS-PAGE) | ||
| Description | UbcH5b is highly homologous to the S. cerevisiae UbC4/5 family of E2 enzymes, demonstrated to be essential for degradation of regulatory and abnormal proteins. UbcH5b, together with UbcH5a and UbcH5c, are the most active class of E2 enzymes in cell extracts and are associated with regulation of a number of transcription factors including p53. Useful for in vitro ubiquitinylation reactions. The His-tagged version of this enzyme is not susceptible to self-ubiquitinylation, which can occur with GST-tagged versions. Typical enzyme concentration to support in vitro conjugation is 100nM-1uM depending upon the conditions. Source: Recombinant protein corresponding to human UbcH5b, fused to His-tag, expressed in E. coli. Molecular Weight: ~18kD Storage and Stability: Aliquot to avoid repeated freezing and thawing and store at -70°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. | ||
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