UBE2E3, Recombinant, Human (UbcH9, Ubiquitin-Conjugating Enzyme E2E 3, Ubiquitin-Conjugating Enzyme E2-23kD, UBCH9, Ubiquitin-Protein Ligase E3, Ubiquitin-Conjugating Enzyme E2E3)

Catalog No : USB-220376
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Product name UBE2E3, Recombinant, Human (UbcH9, Ubiquitin-Conjugating Enzyme E2E 3, Ubiquitin-Conjugating Enzyme E2-23kD, UBCH9, Ubiquitin-Protein Ligase E3, Ubiquitin-Conjugating Enzyme E2E3)
Catalog No USB-220376
Supplier’s Catalog No 220376
Supplier US Biologicals
Source antigen Recombinant
Reactivity
Cross reactivity
Applications
Molecular weight 22.913
Storage -70°C
Other names
Grade Highly Purified
Purity ~95% (RP-HPLC)
Form Supplied as a liquid in 20mM Tris, pH 7.4, 150mM sodium chloride, 10mM DTT, 10% glycerol.
Reactivity life 12 months
Note For reserch purpose only
Purity ~95% (RP-HPLC)
Description Ubiquitin-conjugating enzyme E2 E3 (UBE2E3 alias UbcH9) is a protein that in humans is encoded by the UBE2E3 gene. The deduced 207aa protein shares over 94% aa identity with the UBC domains of class III E2s, UbcH6, UBE2E2, UbcM2, UbcM3, and UbcD2. But the N-terminal extension exhibited little homology among these, except for UbcM2, which showed 100% identity, and which is thought to be a mouse counterpart. The UBE2E3 form a thioester bond with ubiquitin (Ub) in an E1-dependent manner and mediates the transfer of ubiquitin from a ubiquitin-activating enzyme (E1) to a substrate protein or E3 ligase. Source: Recombinant protein corresponding to human UBE2E3. Molecular Weight: ~22.913kD Biological Activity: Protein concentration of 100nM-1uM is recommended for in vitro conjugation. Storage and Stability: Aliquot to avoid repeated freezing and thawing and store at -70°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.