UBE2E3, Recombinant, Human (UbcH9, Ubiquitin-Conjugating Enzyme E2E 3, Ubiquitin-Conjugating Enzyme E2-23kD, UBCH9, Ubiquitin-Protein Ligase E3, Ubiquitin-Conjugating Enzyme E2E3)
Catalog No : USB-220376
556.34€
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| Product name | UBE2E3, Recombinant, Human (UbcH9, Ubiquitin-Conjugating Enzyme E2E 3, Ubiquitin-Conjugating Enzyme E2-23kD, UBCH9, Ubiquitin-Protein Ligase E3, Ubiquitin-Conjugating Enzyme E2E3) | ||
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| Catalog No | USB-220376 | ||
| Supplier’s Catalog No | 220376 | ||
| Supplier | US Biologicals | ||
| Source antigen | Recombinant | ||
| Reactivity | |||
| Cross reactivity | |||
| Applications | |||
| Molecular weight | 22.913 | ||
| Storage | -70°C | ||
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| Other names | |||
| Grade | Highly Purified | ||
| Purity | ~95% (RP-HPLC) | ||
| Form | Supplied as a liquid in 20mM Tris, pH 7.4, 150mM sodium chloride, 10mM DTT, 10% glycerol. | ||
| Reactivity life | 12 months | ||
| Note | For reserch purpose only | ||
| Purity | ~95% (RP-HPLC) | ||
| Description | Ubiquitin-conjugating enzyme E2 E3 (UBE2E3 alias UbcH9) is a protein that in humans is encoded by the UBE2E3 gene. The deduced 207aa protein shares over 94% aa identity with the UBC domains of class III E2s, UbcH6, UBE2E2, UbcM2, UbcM3, and UbcD2. But the N-terminal extension exhibited little homology among these, except for UbcM2, which showed 100% identity, and which is thought to be a mouse counterpart. The UBE2E3 form a thioester bond with ubiquitin (Ub) in an E1-dependent manner and mediates the transfer of ubiquitin from a ubiquitin-activating enzyme (E1) to a substrate protein or E3 ligase. Source: Recombinant protein corresponding to human UBE2E3. Molecular Weight: ~22.913kD Biological Activity: Protein concentration of 100nM-1uM is recommended for in vitro conjugation. Storage and Stability: Aliquot to avoid repeated freezing and thawing and store at -70°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. | ||
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