UBE2R1, Recombinant, Human, His-Tag (CDC34, CDC-34, Ubiquitin-Conjugating Enzyme E2 R1, Ubiquitin-Protein Ligase R1, Ubiquitin-Conjugating Enzyme E2-32kD Complementing, E2-CDC34)

Catalog No : USB-220380
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Product name UBE2R1, Recombinant, Human, His-Tag (CDC34, CDC-34, Ubiquitin-Conjugating Enzyme E2 R1, Ubiquitin-Protein Ligase R1, Ubiquitin-Conjugating Enzyme E2-32kD Complementing, E2-CDC34)
Catalog No USB-220380
Supplier’s Catalog No 220380
Supplier US Biologicals
Source antigen Recombinant
Reactivity
Cross reactivity
Applications
Molecular weight 27.73
Storage -70°C
Other names
Grade Highly Purified
Purity ~95% (RP-HPLC)
Form Supplied as a liquid in 50mM Tris, pH 7.5, 150mM sodium chloride, 10mM DTT, 10% glycerol.
Reactivity life 12 months
Note For reserch purpose only
Purity ~95% (RP-HPLC)
Description UBE2R1 is important in the control of cell cycle and DNA replication. In association with different E3 complexes, including SCF, it has been shown to target many different substrates for ubiquitination and degradation during cell division, signal transduction, and development. UBE2R1 substrates include B-Myb, Wee1, MyoD, ATF5, p27Xic1, and p27Kip1. Additionally, substrates such as p21Cip1, E2F, cyclin E, and cyclin D are putative substrates of UBE2R1 by virtue of their SCF requirement for proteolysis. This protein is useful for in vitro ubiquitinylation reactions. Source: Recombinant protein corresponding to human UBE2R1, fused to His-tag. Molecular Weight: ~27.73kD with His Tag Biological Activity: Protein concentration of 100nM-1uM is recommended for in vitro conjugation. Storage and Stability: Aliquot to avoid repeated freezing and thawing and store at -70°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.